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circ_0005379 inhibits the progression of oral squamous cell carcinoma by regulating the miR-17-5p/acyl-CoA oxidase 1 axis / 华西口腔医学杂志
Article in En | WPRIM | ID: wpr-887754
Responsible library: WPRO
ABSTRACT
OBJECTIVES@#To investigate the effects of circ_0005379 on the proliferation, apoptosis, migration, and invasion of oral squamous cell carcinoma (OSCC) cells and its mechanism.@*METHODS@#Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of circ_0005379 and miR-17-5p in OSCC tissues and SCC15 cell lines. Western blot was used to detect the expression levels of acyl-CoA oxidase 1 (ACOX1). The circ_0005379 overexpression vector was transfected into SCC15 cells. Methyl thiazolyl tetrazolium blue staining, flow cytometry, Transwell, and Western blot were used to detect the effects of circ_0005379 overexpression on the proliferation, apoptosis, migration, and invasion of SCC15 cells and the expression of E-cadherin, β-catenin, and Snail proteins. Dual luciferase reporter assay and RNA immunoprecipitation were used to examine the regulation of circ_0005379, miR-17-5p, miR-17-5p, and ACOX1 in SCC15 cells. A nude mouse xenograft model of SCC15 cells stably overexpressing circ_0005379 was established, and the effect of circ_0005379 overexpression on the growth of xenografts in nude mice was observed.@*RESULTS@#Compared with adjacent cancer tissues, the expression levels of circ_0005379 and ACOX1 proteins in OSCC tissues were decreased (@*CONCLUSIONS@#circ_0005379 may inhibit the proliferation, migration, and invasion of OSCC cells by downregulating the expression of miR-17-5p and upregulating ACOX1, which promote apoptosis and inhibit tumor growth
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Full text: 1 Index: WPRIM Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / MicroRNAs / Acyl-CoA Oxidase / Cell Proliferation / Squamous Cell Carcinoma of Head and Neck / RNA, Circular / Head and Neck Neoplasms / Mice, Nude Limits: Animals / Humans Language: En Journal: West China Journal of Stomatology Year: 2021 Type: Article
Full text: 1 Index: WPRIM Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / MicroRNAs / Acyl-CoA Oxidase / Cell Proliferation / Squamous Cell Carcinoma of Head and Neck / RNA, Circular / Head and Neck Neoplasms / Mice, Nude Limits: Animals / Humans Language: En Journal: West China Journal of Stomatology Year: 2021 Type: Article