Identification and Expression Analysis of TIFY Transcription Factor in Eucommia ulmoides / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 165-174, 2021.
Article
in Zh
| WPRIM
| ID: wpr-906064
Responsible library:
WPRO
ABSTRACT
Objective:
To identify the TIFY gene family in <italic>Eucommia ulmoides</italic> and analyze its expression from the whole genome level to lay the foundation for further study on <italic>EuTIFYs</italic> gene function.Method:
Based on the <italic>E. ulmoides</italic> genome database,the TIFY gene family was identified through bioinformatics analysis tools such as National Center for Biotechnology Information(NCBI),MEME,PlantCare,Expert Protein Analysis System(ExPASy),and TBtools. Physicochemical properties,phylogenetic evolution,gene structure,<italic>cis</italic>-acting elements of the promoters and their expression patterns in leaf development and gum formation of the gene family were systematically analyzed.Result:
In this study,fourteen <italic>EuTIFY</italic> genes (<italic>EuTIFY1</italic>-<italic>EuTIFY14</italic>) were identified in the <italic>E. ulmoides</italic> genome. The <italic>EuTIFYs</italic> were composed of 312-1 074 bp nucleotides encoding 102-357 amino acid residues,with isoelectric points of 4.99-10.06 and molecular weight in the range of 10.8-39.14 kDa. According to putative subcellular localization,proteins,which were mainly hydrophilic proteins,localized in the nucleus. The 14 <italic>EuTIFYs</italic> were unevenly distributed on 13 chromosomes. <italic>EuTIFY</italic> gene family was divided into four subfamilies: <italic>TIFY</italic>,<italic>JAZ</italic>,<italic>ZML</italic>,and <italic>PPD</italic>,which contained three,four,five,and two members respectively. The promoters of <italic>EuTIFYs</italic> contained multiple photoperiodic and abiotic stress-responsive cis-acting elements,which were involved in plant growth and abiotic stress regulation. Expression pattern analysis showed that <italic>EuTIFYs </italic>exhibited different expression levels in different development stages of <italic>E. ulmoides</italic> leaves and multiple interactions,and most of the genes were highly expressed in the early stage of leaf development and positively regulated the formation of <italic>E. ulmoides</italic> gum.Conclusion:
Fourteen <italic>EuTIFYs</italic> were identified from the whole genome of <italic>E. ulmoides</italic>,and their structural characteristics and expression patterns were analyzed by bioinformatics. The findings of this study are expected to provide references for further research on the function of <italic>EuTIFYs</italic>.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
Zh
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2021
Type:
Article