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Analysis of Chemical Constituents in Daishenning by HPLC-Q-TOF-MS/MS / 中国实验方剂学杂志
Article in Chinese | WPRIM | ID: wpr-906310
Responsible library: WPRO
ABSTRACT

Objective:

High performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometryHPLC-Q-TOF-MS/MS) was used to identify the main chemical constituents of Daishenning.

Method:

Cosmosil 5 C<sub>18</sub>-AR-Ⅱ column (4.6 mm×250 mm, 5 μm) was employed for chromatographic separation with mobile phase of acetonitrile (A)-0.5% formic acid aqueous solution (B) for gradient elution (0-10 min, 5%A; 10-20 min, 5%-20%A; 20-30 min, 20%A; 30-55 min, 20%-35%A; 55-65 min, 35%-55%A; 65-75 min, 55%-100%A; 75-80 min, 100%A; 80-85 min, 100%-5%A; 85-90 min, 5%A), the flow rate was 1 mL·min<sup>-1</sup>, column temperature was 40 ℃, and injection volume was 10 μL. Electrospray ionization (ESI), positive and negative ion detection modes and mass scanning range of <italic>m</italic>/<italic>z</italic> 100-2 000 were selected for mass spectrometry. The main chemical constituents in Daishenning were identified by MassHunter B.06.00 software in combination with PubChem, MassBank, ChemicalBook and other databases, and reference information.

Result:

A total of 96 components were identified from Daishenning, including 32 flavonoids, 19 organic acids, 6 glycosides, 6 terpenoids, 5 phenylpropanoids, 8 phenols, 14 other components and 6 unknown components.

Conclusion:

The established method can simultaneously analyze different types of compounds in Daishenning, it is helpful for further research on the extraction and separation of main chemical components and quality control of this preparation. In addition, through the rapid identification of the chemical constituents in Daishenning, it is speculated that the main effective substances of Daishenning may be flavonoids and organic acids.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2021 Type: Article