Lipid Accumulation and IL-6 Production in L02 Hepatocytes Induced by Sodium Oleate: Dose and Time Dependence / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 998-1004, 2021.
Article
in En
| WPRIM
| ID: wpr-921358
Responsible library:
WPRO
ABSTRACT
To explore interleukin-6 (IL-6) production and characterize lipid accumulation in L02 hepatocytes induced by sodium oleate. L02 hepatocytes were incubated with 0, 37.5, 75, 150, 300, 600, or 1,200 μmol/L sodium oleate for 24 h, and the supernatant was collected to detect the concentration of IL-6. L02 hepatocytes were incubated with 300, 150, 75, or 0 μmol/L sodium oleate for 0-24 h. The supernatant was collected for detection of IL-6 and free fatty acids. L02 hepatocytes treated with 300 μmol/L sodium oleate for 0-24 h were stained with Oil Red O. With extended sodium oleate incubation time, IL-6 levels increased, and free fatty acids decreased. After 24 h incubation, IL-6 levels increased as sodium oleate increased from 37.5 to 300 μmol/L (
Key words
Full text:
1
Index:
WPRIM
Main subject:
Time Factors
/
Interleukin-6
/
Oleic Acid
/
Hepatocytes
/
Dose-Response Relationship, Drug
/
Lipid Metabolism
Limits:
Humans
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2021
Type:
Article