Analysis on ingredients and metabolites of Gegen Decoction absorbed into blood based on UHPLC-Q-TOF-MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 5944-5952, 2021.
Article
in Zh
| WPRIM
| ID: wpr-921717
Responsible library:
WPRO
ABSTRACT
This study analyzed the plasma components of Gegen Decoction(GGD) by ultra-high-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS), which is expected to serve as a reference for exploring the pharmacodynamic substances of GGD. Female Wistar rats were given(ig) GGD and then plasma samples were collected and analyzed by UHPLC-Q-TOF-MS. The results showed that 42 chemical components were identified 25 prototypes(14 from Puerariae Lobatae Radix, 6 from Glycyrrhizae Radix et Rhizoma, 3 from Paeoniae Radix Alba, and 2 from Ephedrae Herba) and 17 metabolites(from isoflavonoids in Puerariae Lobatae Radix and Glycyrrhizae Radix et Rhizoma). UHPLC-Q-TOF-MS was employed to achieve rapid analysis of plasma components of GGD, laying a basis for elucidating the therapeutic material basis and mechanism of GGD.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Mass Spectrometry
/
Drugs, Chinese Herbal
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Chromatography, High Pressure Liquid
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Rats, Wistar
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Paeonia
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2021
Type:
Article