Optimization of extraction technology of volatile oil from Blumea balsamifera and analysis on its compositions / 中国药房
China Pharmacy
; (12): 950-956, 2022.
Article
in Zh
| WPRIM
| ID: wpr-923597
Responsible library:
WPRO
ABSTRACT
OBJECTIVE To opti mize the supercritical CO 2 extraction technology of volatile oil from Blumea balsamifera ,and compare the components of the volatile oil from B. balsamifera obtained by supercritical CO 2 extraction and steam distillation. METHODS The volatile oil of B. balsamifera was extracted by supercritical CO 2 extraction. Using extraction rate of volatile oil as index,extraction temperature ,extraction pressure and extraction time as factors ,based on single-factor experiment ,orthogonal experiment was used to optimize the supercritical CO 2 extraction technology. Gas chromatography-mass spectrometry was used to identify the components of volatile oil from B. balsamifera . Peak area normalization was used to calculate the relative contents of each component. Taking the volatile oil obtained by steam distillation as a reference ,the extraction rates ,components and contents of volatile oil by the two methods were compared. RESULTS The optimal supercritical CO 2 extraction technology of volatile oil from B. balsamifera included extraction pressure of 30 MPa,extraction temperature of 50 ℃ and extracting for 50 min. After 3 times of validation tests ,average extraction rate of volatile oil was 4.64%(RSD=0.54%,n=3). Thirty-nine components such as tritriacontane,stigmasterol,squalene were identified in the volatile oil of B. balsamifera obtained by supercritical CO 2 extraction; and 51 components such as triacontane ,ledol,humulene epoxide Ⅰ were identified by steam distillation. The extraction rate of volatile oil from B. balsamifera obtained by 2 methods were 4.64% and 0.99%. A total of 26 common components were obtained , such as xanthoxylin ,L-borneol,β-caryophyllene. Except for xanthoxyline (34.829% by supercritical CO 2 extraction,30.676% by steam distillation method )and phytol (2.401% by supercritical CO 2 extraction,1.273% by steam distillation ),the relative contents of the components of volatile oil obtained by supercritical CO 2 extraction were lower than those of steam distillation. CONCLUSIONS The optimal supercritical CO 2 extraction technology is stable and feasible ;the components and contents of volatile oil obtained by two methods varies greatly ,and main compounds are aldehydes and ketones ,alkenes,alcohols and other components.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2022
Type:
Article