~1H-qNMR quantification of total ginsenosides in Shenmai Injection / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 587-592, 2022.
Article
in Zh
| WPRIM
| ID: wpr-927940
Responsible library:
WPRO
ABSTRACT
A content determination method based on ~1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L~(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Quality Control
/
Drugs, Chinese Herbal
/
Ginsenosides
/
Drug Combinations
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2022
Type:
Article