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Establishment of a platelet production model by bone marrow cavity transplantation of mouse primary megakaryocytes / 中华血液学杂志
Chinese Journal of Hematology ; (12): 272-278, 2022.
Article in Zh | WPRIM | ID: wpr-929635
Responsible library: WPRO
ABSTRACT
Objective: To establish an intramedullary transplantation model of primary megakaryocytes to evaluate the platelet-producing capacity of megakaryocytes and explore the underlying regulatory mechanisms. Methods: Donor megakaryocytes from GFP-transgenic mice bone marrow were enriched by magnetic beads. The platelet-producing model was established by intramedullary injection to recipient mice that underwent half-lethal dose irradiation 1 week in advance. Donor-derived megakaryocytes and platelets were detected by immunofluorescence staining and flow cytometry. Results: The proportion of megakaryocytes in the enriched sample for transplantation was 40 to 50 times higher than that in conventional bone marrow. After intramedullary transplantation, donor-derived megakaryocytes successfully implanted in the medullary cavity of the recipient and produce platelets, which showed similar expression of surface markers and morphology to recipient-derived platelets. Conclusion: We successfully established an in vivo platelet-producing model of primary megakaryocytes using magnetic-bead enrichment and intramedullary injection, which objectively reflects the platelet-producing capacity of megakaryocytes in the bone marrow.
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Full text: 1 Index: WPRIM Main subject: Blood Platelets / Bone Marrow / Bone Marrow Cells / Megakaryocytes / Bone Marrow Transplantation Limits: Animals / Humans Language: Zh Journal: Chinese Journal of Hematology Year: 2022 Type: Article
Full text: 1 Index: WPRIM Main subject: Blood Platelets / Bone Marrow / Bone Marrow Cells / Megakaryocytes / Bone Marrow Transplantation Limits: Animals / Humans Language: Zh Journal: Chinese Journal of Hematology Year: 2022 Type: Article