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The in vivo imaging of zebrafish for chemical vascular toxicity assessment / 中华预防医学杂志
Article in Zh | WPRIM | ID: wpr-935247
Responsible library: WPRO
ABSTRACT
Objective: To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish. Methods: The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively. Results: The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions. Conclusion: Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Zebrafish / Animals, Genetically Modified / Cardiovascular System / Toxicity Tests Limits: Animals Language: Zh Journal: Chinese Journal of Preventive Medicine Year: 2022 Type: Article
Full text: 1 Index: WPRIM Main subject: Zebrafish / Animals, Genetically Modified / Cardiovascular System / Toxicity Tests Limits: Animals Language: Zh Journal: Chinese Journal of Preventive Medicine Year: 2022 Type: Article