The in vivo imaging of zebrafish for chemical vascular toxicity assessment / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 33-37, 2022.
Article
in Zh
| WPRIM
| ID: wpr-935247
Responsible library:
WPRO
ABSTRACT
Objective: To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish. Methods: The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively. Results: The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions. Conclusion: Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Full text:
1
Index:
WPRIM
Main subject:
Zebrafish
/
Animals, Genetically Modified
/
Cardiovascular System
/
Toxicity Tests
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Preventive Medicine
Year:
2022
Type:
Article