The Effect of hnRNPK/Beclin1 Signaling on the Drug Resistance of Imatinib in Ph+ Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 750-754, 2022.
Article
in Zh
| WPRIM
| ID: wpr-939683
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of hnRNPK/Beclin1 signaling on the drug resistance of imatinib in Ph+ leukemia.@*METHODS@#Expression level of hnRNPK was verified in the imatinib resistant and sensitive Ph+ leukemia cell lines by using Western blot. hnRNPK expression was down-regulated by using RNAi. Expression level of LC3I/II and Beclin1 were detected by Western blot and the sensitivity of imatinib was analyzed by CCK-8 assay before and after modulation of hnRNPK expression.@*RESULTS@#hnRNPK showed overexpressed in imatinib resistant leukemia cell line. After the expression level of hnRNPK was down-regulated by RNAi, the sensitivity of drug resistance lines to imatinib restored, while the expression level of LC3I/II and Beclin1 were consistant with the modulation of hnRNPK expression.@*CONCLUSION@#hnRNP K/Beclin1 signaling may be involved in the development of imatinib resistance in Ph+ leukemia through the regulation of autophagy.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Drug Resistance
/
Leukemia
/
Drug Resistance, Neoplasm
/
Heterogeneous-Nuclear Ribonucleoprotein K
/
Cell Line, Tumor
/
Imatinib Mesylate
/
Beclin-1
/
Antineoplastic Agents
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2022
Type:
Article