Comparison of cell growth suppression in SiHa cervical carcinoma cell line by human papillomavirus type 16 E6/E7 siRNAs / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
; : 35-42, 2010.
Article
in Ko
| WPRIM
| ID: wpr-9572
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
Human cervical cancer is caused by the high-risk types of human papillomavirus (HPV) such as HPV16, which possess the E6 and E7 oncogenes, whose expressions are a prerequisite for cancer development. We performed this study to compare the efficacy of antitumor activity by HPV siRNA which silences only E6 or both E6/E7.METHODS:
We transfected siRNA 377 (HPV16 E6 siRNA), siRNA 3 (HPV16 E6 siRNA), and siRNA 198 (HPV16 E7 siRNA) into SiHa cell line (siRNA 377 silences only E6, and siRNA 3 and siRNA 198 silence both E6 and E7). We experimented cell counts and morphologic changes 24 and 48 hours after transfection and expressions of HPV16 E6/E7 mRNA by RT-PCR.RESULTS:
siRNA 377, siRNA 3, and siRNA 198 suppressed the cell growth. siRNA 3 and siRNA 198 were more potent than siRNA 377 in cell growth suppression. siRNA 377 knocked down the expression of E6 mRNA, and both siRNA 3 and siRNA 198 knocked down the expression of E6/E7 mRNA.CONCLUSION:
Our results suggest that simultaneous suppression of E6 and E7 was more potent than E6-specific suppression in cancer cell growth.Key words
Full text:
1
Index:
WPRIM
Main subject:
Oncogenes
/
RNA, Messenger
/
Transfection
/
Cell Count
/
Cell Line
/
Uterine Cervical Neoplasms
/
RNA, Small Interfering
Limits:
Humans
Language:
Ko
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2010
Type:
Article