Effects of L-Arginine and 7-Nitroindazole in the Gerbil Model of Global Cerebral Ischemia
Journal of the Korean Society of Emergency Medicine
; : 23-28, 2003.
Article
in Ko
| WPRIM
| ID: wpr-97141
Responsible library:
WPRO
ABSTRACT
PURPOSE: This study was performed to determine whether L-Arginine would improve or worsen the neurologic out-come after ischemic brain injury and whether 7- Nitroindazole (7-NI, inhibitors of neuronal nitric oxide (NO) synthase inhibitor) would improve or worsen. METHODS: Five (5) groups (N =11 to 14) of anesthetized gerbils were subjected to 10 min of global cerebral ischemia by means of a bilateral carotid artery occlusion. One group (N =11) was the control group. In a second group (N =12), the animals were pretreated with intraperitoneal L-Arginine (300 mg/kg) one hour before ischemic insult. In a third group (N =12), pretreatment with L-Arginine was performed in the same manner and intraperitoneal 7-NI were given at the time of reperfusion and 2 hours after reperfusion. The animals of a fourth group (N =12) were treated with 7-NI in the same manner without any pretreatment with L-Arginine. The animals in the last group (N =14) underwent sham operations. RESULTS: Compared with control group, concomittent treatment with L-Arginine and 7-NI showed no significant improvement in the neurological cell survival rate. Also, group pretreated with L-Arginine only showed a similar out-come. The group treated with 7-NI at the time of reperfusion and 2 hours after reperfusion showed a significant improvement in the neurological cell survival rate compared with the control group and the other two experimental groups (p<0.05). CONCLUSION: In the ischemia-reperfusion injury of global ischemia in gerbils, NO from endothelial NO synthase has no important role in the neurological outcome.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Arginine
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Brain Injuries
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Carotid Arteries
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Reperfusion
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Reperfusion Injury
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Cell Survival
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Brain Ischemia
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Gerbillinae
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Nitric Oxide Synthase
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Ischemia
Limits:
Animals
Language:
Ko
Journal:
Journal of the Korean Society of Emergency Medicine
Year:
2003
Type:
Article