Impaired angiogenesis in the enalapril-treated neonatal rat kidney / 소아과
Korean Journal of Pediatrics
;
: 8-15, 2016.
Article
in English
| WPRIM
| ID: wpr-98143
ABSTRACT
PURPOSE:
Nephrogenesis is normally accompanied by a tightly regulated and efficient vascularization. We investigated the effect of angiotensin II inhibition on angiogenesis in the developing rat kidney.METHODS:
Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle (control) for 7 days after birth. Renal histological changes were checked using Hematoxylin & Eosin staining. We also investigated the intrarenal expression of vascular endothelial growth factor (VEGF)-A, VEGF receptor 1 (VEGFR1), VEGFR2, platelet-derived growth factor (PDGF)-B, and PDGF receptor-beta with Western blotting and immunohistochemical staining at postnatal day 8. Expression of the endothelial cell marker CD31 was examined to determine glomerular and peritubular capillary density.RESULTS:
Enalapril-treated rat kidneys showed disrupted tubules and vessels when compared with the control rat kidneys. In the enalapril-treated group, intrarenal VEGF-A protein expression was significantly higher, whereas VEGFR1 protein expression was lower than that in the control group (P<0.05). The expression of VEGFR2, PDGF-B, and PDGF receptor-beta was not different between the 2 groups. The increased capillary CD31 expression on the western blots of enalapril-treated rat kidneys indicated that the total endothelial cell protein level was increased, while the cortical capillary density, assessed using CD31 immunohistochemical staining, was decreased.CONCLUSION:
Impaired VEGF-VEGFR signaling and altered capillary repair may play a role in the deterioration of the kidney vasculature after blocking of angiotensin II during renal development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Platelet-Derived Growth Factor
/
Capillaries
/
Angiotensin II
/
Enalapril
/
Blotting, Western
/
Eosine Yellowish-(YS)
/
Receptors, Vascular Endothelial Growth Factor
/
Parturition
/
Endothelial Cells
/
Vascular Endothelial Growth Factor A
Limits:
Animals
/
Humans
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2016
Type:
Article
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