Mechanism of Der f 1/IGF-1 nanoparticle promoting the production of regulatory T cell / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (12): 272-277, 2023.
Article
in Zh
| WPRIM
| ID: wpr-982731
Responsible library:
WPRO
ABSTRACT
Objective:
To prepare PLGA nanoparticles loaded with Der f 1/IGF-1(Der f 1/IGF-1 NPs) and investigate their role in promoting the formation of Treg cells.Methods:
NPs coated with Der f 1/IGF-1 were prepared by double emulsion method and their physicochemical properties and cumulative release rate in vitro were analyzed. After pretreatment, BMDC was divided into Saline group, Blank NPs group, Der f 1/IGF-1 group and Der f 1/IGF-1 NPs group. Determination of the expression of IL-10 and TGF-β in BMDC by ELISA. The number of Treg cells was detected by flow cytometry.Results:
The results showed that Der f 1/IGF-1 NPs were spherical structures, with good dispersion, particle size less than 200 nm, negative charge and stable slow-release effect of Zeta potential. After BMDC pretreatment, the expression levels of TGF-β and IL-10 in BMDC cells in the Der f 1/IGF-1 NPs group were significantly increased compared with the Blank NPs group, and the difference was statistically significant(P<0.001). After co-culture with CD4+ T cells, the proportion of Treg cells produced in the Der f 1/IGF-1 NPs group was significantly increased, and the difference was statistically significant(P<0.001).Conclusion:
Der f 1/IGF-1 NPs can induce Treg cell generation in vitro. This study provides a new and more effective method for the reconstruction of immune tolerance dysfunction.Key words
Full text:
1
Index:
WPRIM
Main subject:
Particle Size
/
Insulin-Like Growth Factor I
/
Drug Carriers
/
Transforming Growth Factor beta
/
Interleukin-10
/
T-Lymphocytes, Regulatory
/
Nanoparticles
Limits:
Humans
Language:
Zh
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2023
Type:
Article