Concentration and determination of strychnine alkaloid in biological fluids / 法医学杂志
Journal of Forensic Medicine
; (6): 36-38, 2005.
Article
in Zh
| WPRIM
| ID: wpr-983070
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers.@*METHODS@#A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids.@*RESULTS@#the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit.@*CONCLUSION@#The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.
Full text:
1
Index:
WPRIM
Main subject:
Polymers
/
Strychnine
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Alkaloids
Limits:
Animals
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2005
Type:
Article