Time-dependent expression of astroglial S100beta following diffuse brain injury in rats / 法医学杂志
Journal of Forensic Medicine
; (6): 4-6, 2006.
Article
in Zh
| WPRIM
| ID: wpr-983115
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the dynamics of the induction of S100beta in different parts of rat brain following the diffuse brain injury.@*METHODS@#Immunohistochemistry and auto-image analysis were to determine the expression of astroglial S100beta after diffuse brain injury in rats. Forty rats were distributed into groups according to injury time of 30min, and2,4,12,24h, and 3,6 d after diffuse brain injury, and normal rats as control.@*RESULTS@#The number of S100beta positive cells in the four areas increased significantly followed by a decrease, and then a further increase. The expression of S100beta could be detected increasing in 2h, and increased significantly in 4h, and it reached apex 12h after DBI, and decreased gradually to the level less than normal 3d, and returned to normal 7d following injury. In the postmortem injury groups, there were no significant changes in anti-S100beta immunoreactivities in four areas of brain compared to the control group.@*CONCLUSION@#The present study showed the time-dependent expression of S100beta is obvious following diffuse brain injury, and suggested S100beta be suitable as a marker for brain injury age determination.
Full text:
1
Index:
WPRIM
Main subject:
Staining and Labeling
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Time Factors
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Brain
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Brain Edema
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Brain Injuries
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Immunohistochemistry
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S100 Proteins
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Random Allocation
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Neuroglia
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Rats, Sprague-Dawley
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article