Expression of HIF1-alpha on myocardium and lung in rats model of asphyxia death / 法医学杂志
Journal of Forensic Medicine
; (6): 407-410, 2006.
Article
in Zh
| WPRIM
| ID: wpr-983234
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the expression of HIF1-alpha in heart and lung tissue died from asphyxia.@*METHODS@#The rats model of asphyxia death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time (0,2,6,24 h), immunohistochemistry and half-quantitative RT-PCR methods were used to investigate expression of HIF1-alpha and mRNA changes on heart and lung tissue.@*RESULTS@#The positive staining of HIF1-alpha could be observed in the myocardium and lung tissue. Significant differences were found between the groups of asphyxia and their corresponding control group. HIF1-alpha expression was found in all the asphyxia groups while it was only expressed in the control groups of 2 h, 6 h and 24 h. Nucleic positive staining could be detected in all the asphyxia groups but none was found in the control groups. RT-PCR showed that the expression of mRNA between 0 h asphyxia group and 0 h control group were equal in both cardic muscle and lung, but elevated expression in groups of 2,6,24h compared to their control groups.@*CONCLUSION@#The nuclear positive staining of HIF1-alpha in heart and lung can be a special character of suffocation death.
Full text:
1
Index:
WPRIM
Main subject:
Asphyxia
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Time Factors
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RNA, Messenger
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Immunohistochemistry
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Tissue Distribution
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Random Allocation
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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Hypoxia-Ischemia, Brain
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Disease Models, Animal
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2006
Type:
Article