Determination of ethyl glucuronide in blood by GC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 431-433, 2009.
Article
in Zh
| WPRIM
| ID: wpr-983519
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).@*METHODS@#Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.@*RESULTS@#The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.@*CONCLUSION@#The method is suitable for blood EtG analysis.
Full text:
1
Index:
WPRIM
Main subject:
Alcohol Drinking
/
Biomarkers
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Alcoholism
/
Forensic Toxicology
/
Glucuronates
/
Gas Chromatography-Mass Spectrometry
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2009
Type:
Article