Determination of TTX in biological samples by LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 432-435, 2010.
Article
in Zh
| WPRIM
| ID: wpr-983607
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).@*METHODS@#Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.@*RESULTS@#The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.@*CONCLUSION@#This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.
Full text:
1
Index:
WPRIM
Main subject:
Specimen Handling
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Swine
/
Tetrodotoxin
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Body Fluids
/
Reproducibility of Results
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Chromatography, Liquid
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Sensitivity and Specificity
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Tandem Mass Spectrometry
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Solid Phase Extraction
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Forensic Toxicology
Limits:
Animals
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Female
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Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2010
Type:
Article