Detection of matrix metalloproteinase-11 in menstrual blood by enhanced chemiluminescence method / 法医学杂志
Journal of Forensic Medicine
; (6): 109-111, 2012.
Article
in Zh
| WPRIM
| ID: wpr-983722
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method.@*METHODS@#Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.@*RESULTS@#The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 microL sample was added, the mass concentration of protein was 1.329 microg/microL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 degrees C for 20 months.@*CONCLUSION@#Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
Full text:
1
Index:
WPRIM
Main subject:
Saliva
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Urine
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Vagina
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Blood Stains
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Biomarkers
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Blotting, Western
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Reproducibility of Results
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Sensitivity and Specificity
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Matrix Metalloproteinase 11
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Forensic Medicine
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2012
Type:
Article