LC-MS/MS analysis of determination of strychnine and brucine in formaldehyde fixed tissue / 法医学杂志
Journal of Forensic Medicine
; (6): 347-350, 2012.
Article
in Zh
| WPRIM
| ID: wpr-983759
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish a method for determination of strychnine and brucine in formaldehyde fixed tissue by LC-MS/MS analysis.@*METHODS@#The samples were pretreated with solid phase extraction using SCX cartridges and separated on SB-C18 column with mobile phase 0.1% formic acid 0.1% formic acid-acetonitrile (7525). Electrospray ionization (ESI) source was utilized and operated in positive ion mode. Multiple reactions monitoring (MRM) mode was applied. External standard method was applied for quantitation.@*RESULTS@#The chromatographic separation of strychnine and brucine in formaldehyde fixed nephritic and hepatic tissues resulted successfully. The standard curve was linear in the range of 0.002-2.0 microg/g for strychnine and brucine in formaldehyde fixed tissues, and the correlation coefficient was more than 0.996. The limits of detection (LOD) of strychnine and brucine in nephritic tissues were 0.06ng/g and 0.03 ng/g, respectively. The LOD of both chemicals were 0.3 ng/g in hepatic tissues. The extraction recovery rate was more than 74.5%. The precision of intra-day and inter-day were both less than 8.2%.@*CONCLUSION@#Strychnine and brucine can be sensitive to be determined in formaldehyde fixed tissue by LC-MS/MS analysis. It can be applied in the forensic toxicological analysis.
Full text:
1
Index:
WPRIM
Main subject:
Mass Spectrometry
/
Strychnine
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Tissue Distribution
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Molecular Structure
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Reproducibility of Results
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Chromatography, Liquid
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
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Forensic Toxicology
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Limit of Detection
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2012
Type:
Article