Determination of the biological attribute of evidence at the scene using reverse transcription PCR and real-time fluorescent quantitative PCR / 法医学杂志
Journal of Forensic Medicine
;
(6): 259-262, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-983832
ABSTRACT
OBJECTIVE@#To explore the feasibility of biological method to identify the biological attribute of samples at crime scene.@*METHODS@#Thirty samples of ten blood stains, ten saliva stains and ten semen stains were selected, and all the samples were processed by the routine method and biomolecular method, respectively. Both RNA and DNA were isolated using DNA-RNA co-extraction technology and the mRNA was converted into cDNA using reverse transcription PCR (RT-PCR). Three pairs of specific primers were designed for blood stain, saliva stain and semen stain based on the different target genes in three specific tissues and the primers were amplified using real-time fluorescent quantitative PCR. The differences in these biological samples were evaluated by melting temperature (Tm) values and the size of the amplification fragment.@*RESULTS@#The Tm values of blood stain, saliva stain and semen stain were (84.5 +/- 0.2) degrees C, (76.9 +/- 0.3) degrees C and (88.5 +/- 0.2) degrees C, respectively. The length of PCR fragments of them was 177bp, 134bp and 294bp, respectively.@*CONCLUSION@#Compared with the routine method, RT-PCR with real-time fluorescent quantitative PCR is highly specific, sensitive and reliable to identify the biological attribute of evidence, and can be potentially applied to determine evidence attribute in forensic practice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saliva
/
Semen
/
Blood Stains
/
DNA
/
RNA
/
RNA, Messenger
/
Genetic Markers
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
DNA Primers
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2013
Type:
Article
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