Relationship between PMI and relative expression of myocardial various RNAs in rats died of different causes / 法医学杂志
Journal of Forensic Medicine
; (6): 7-12, 2014.
Article
in Zh
| WPRIM
| ID: wpr-983871
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the changes of relative expression of myocardial various RNAs in rats died of different causes and their relationship with PMI.@*METHODS@#The rat models were established in which the rats were sacrificed by broken neck, asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium. The quantitative real time PCR was used to calculate threshold cycle values of RNAs including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, inducible nitric oxide synthase (iNOS), hypoxia-inducible factor-1 (HIF-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and U6 small nuclear RNA (U6 snRNA) and to study the changes of the relative expressions of various indexes with PMI.@*RESULTS@#U6 snRNA with stable expression level could be used as appropriate internal control. In the early PMI, the relative expression of GAPDH, HIF-1, iNOS, TNF-alpha, and IL-6 more characteristically increased in groups of asphyxia and hemorrhagic shock than in group of broken neck, but the quantity of beta-actin decreased in all groups. In the late PMI, all the relative expressions significantly declined in correlation with the degradation of RNA.@*CONCLUSION@#The characteristic changes of each RNA expression can be used as references to estimate PMI in deaths by different causes.
Full text:
1
Index:
WPRIM
Main subject:
Shock, Hemorrhagic
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RNA
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RNA, Small Nuclear
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Cause of Death
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Cytokines
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Actins
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Tumor Necrosis Factor-alpha
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Disease Models, Animal
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Enzymes
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Nitric Oxide Synthase Type II
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2014
Type:
Article