Cathepsin L expression in plasma after acute myocardial ischemia and ischemia-reperfusion in rats / 法医学杂志
Journal of Forensic Medicine
; (6): 253-256, 2014.
Article
in Zh
| WPRIM
| ID: wpr-983912
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To test cathepsin L as a biomarker of myocardial ischemia by examination of cathepsin L expression in plasma after myocardial ischemia and ischemia-reperfusion in rats.@*METHODS@#The rat models were established and divided in acute myocardial ischemia model (myocardial ischemia 30 min, 1 h, 2 h groups), ischemia-reperfusion model (ischemia-reperfusion group), and isoflurane-pretreated ischemia-reperfusion model (isoflurane-pretreated group), respectively. Normal control group and sham-operated group were established as contrast. The contents of cathepsin L in plasma were examined by ELISA and myocardial infarction areas were measured after TTC staining.@*RESULTS@#No statistical significant changes were found among the experimental groups compared with the normal control group and sham-operated group (P>0.05). The cathepsin L from the ischemia-reperfusion group increased to 2.37 times compared with the normal control group (P<0.05). The cathepsin L and myocardium infarction size of isoflurane-pretreated group decreased compared with the ischemia-reperfusion group (P<0.05).@*CONCLUSION@#The cathepsin L in plasma is not a promising biomarker of acute myocardial ischemia. Isoflurane preconditioning can reduce the cathepsin L in plasma caused by ischemia-reperfusion injury.
Full text:
1
Index:
WPRIM
Main subject:
Biomarkers
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Myocardial Reperfusion Injury
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Myocardial Ischemia
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Cathepsin L
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Isoflurane
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Myocardial Infarction
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Myocardium
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2014
Type:
Article