High-efficiency preparation technology of sulforaphane: an overview

ABSTRACT

Sulforaphane is an isothiocyanate metabolite of cruciferous plants, which obtain antioxidant, anticancer and anti-COVID-19 functions. However, due to its unstable structure, it is easy to de-composite, thus the utilization of sulforaphane is difficult. With the advancement of the preparation of sulforaphane, the purpose of inhibiting sulforaphane inactivation and improving its utilization is expected to be realized. The existing preparation technologies are mainly myrosinase enzymatic hydrolysis, microbial transformation and chemical synthesis. Myrosinase enzymatic hydrolysis mainly utilizes endogenous myrosinase, exogenous myrosinase and heterologously expressed myrosinase. Myrosinase enzymatic hydrolysis technology not only obtain the advantage of high preparation efficiency, but also obtain the disadvantage that the activity of myrosinase cannot be stabilized. Microbial transformation mainly utilizes the function of microorganisms to convert glucosinolates to sulforaphane, and obtain the advantages of easy control of reaction conditions and low cost. Chemical synthesis mainly includes de novo synthesis and semi-synthesis, and semi-synthesis is the most widely used method at present. Chemical synthesis obtains the advantages of easy control of reaction conditions, but chemical synthesis techniques have the problems of high risk and low yield. This research reviews the preparation technology of sulforaphane, aiming to provide a reference for the efficient utilization of sulforaphane and its product development.