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Vulnerabilities in coronavirus glycan shields despite extensive glycosylation.
Watanabe, Yasunori; Berndsen, Zachary T; Raghwani, Jayna; Seabright, Gemma E; Allen, Joel D; Pybus, Oliver G; McLellan, Jason S; Wilson, Ian A; Bowden, Thomas A; Ward, Andrew B; Crispin, Max.
  • Watanabe Y; School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
  • Berndsen ZT; Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
  • Raghwani J; Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK.
  • Seabright GE; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Allen JD; Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7LF, UK.
  • Pybus OG; School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
  • McLellan JS; Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
  • Wilson IA; School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
  • Bowden TA; Department of Zoology, University of Oxford, Oxford, OX1 3PS, UK.
  • Ward AB; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712, USA.
  • Crispin M; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
Nat Commun ; 11(1): 2688, 2020 05 27.
Article in English | MEDLINE | ID: covidwho-432476
Preprint
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Semantic information from SemMedBD (by NLM)
1. envelope PART_OF HIV-1
Subject
envelope
Predicate
PART_OF
Object
HIV-1
2. glycoprotein complex PART_OF Lassa virus
Subject
glycoprotein complex
Predicate
PART_OF
Object
Lassa virus
3. Hemagglutinin INTERACTS_WITH Epitopes
Subject
Hemagglutinin
Predicate
INTERACTS_WITH
Object
Epitopes
4. Polysaccharides compared_with Viral Proteins
Subject
Polysaccharides
Predicate
compared_with
Object
Viral Proteins
5. envelope PART_OF HIV-1
Subject
envelope
Predicate
PART_OF
Object
HIV-1
6. glycoprotein complex PART_OF Lassa virus
Subject
glycoprotein complex
Predicate
PART_OF
Object
Lassa virus
7. Hemagglutinin INTERACTS_WITH Epitopes
Subject
Hemagglutinin
Predicate
INTERACTS_WITH
Object
Epitopes
8. Polysaccharides compared_with Viral Proteins
Subject
Polysaccharides
Predicate
compared_with
Object
Viral Proteins
ABSTRACT
Severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) are zoonotic pathogens with high fatality rates and pandemic potential. Vaccine development focuses on the principal target of the neutralizing humoral immune response, the spike (S) glycoprotein. Coronavirus S proteins are extensively glycosylated, encoding around 66-87 N-linked glycosylation sites per trimeric spike. Here, we reveal a specific area of high glycan density on MERS S that results in the formation of oligomannose-type glycan clusters, which were absent on SARS and HKU1 CoVs. We provide a comparison of the global glycan density of coronavirus spikes with other viral proteins including HIV-1 envelope, Lassa virus glycoprotein complex, and influenza hemagglutinin, where glycosylation plays a known role in shielding immunogenic epitopes. Overall, our data reveal how organisation of glycosylation across class I viral fusion proteins influence not only individual glycan compositions but also the immunological pressure across the protein surface.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Polysaccharides / Glycoproteins / Viral Fusion Proteins / Spike Glycoprotein, Coronavirus / Middle East Respiratory Syndrome Coronavirus Topics: Vaccines Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-16567-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Polysaccharides / Glycoproteins / Viral Fusion Proteins / Spike Glycoprotein, Coronavirus / Middle East Respiratory Syndrome Coronavirus Topics: Vaccines Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-16567-0