RESUMO
Emilio Ribas, an administrative physician and sanitarian from Sao Paulo, was an advocate of the microbiological conception during the long period when he directed the Servico Sanitario de Sao Paulo (1898-1917). This article offers a brief analysis of his stance in the flight against yellow fever during the early 20th century, considered a highlight of his career. The scientists and professionals linked to this sanitary agency in Sao Paulo found themselves in a tense, unstable work environment, where experimental proof played an important role in corroborating new medical-sanitary conceptions and practices. The article takes a close look at the medical experiments on yellow fever directed by Emilio Ribas and conducted at Sao Paulo's Hospital de Isolamento in 1902-03. It examines these same studies in an effort to better understand how the significance of yellow fever was transformed and to explore its implications regarding the history of public health in Sao Paulo.
Assuntos
Controle de Doenças Transmissíveis/história , Saúde Pública/história , Pesquisa/história , Febre Amarela/história , Brasil , História do Século XIX , História do Século XXRESUMO
The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form.
Assuntos
Proteínas de Bactérias/genética , Glicosilfosfatidilinositóis/genética , Mycobacterium leprae/imunologia , Proteínas de Saccharomyces cerevisiae , Proteínas de Bactérias/imunologia , Proteínas Fúngicas/genética , Genes Fúngicos , Vetores Genéticos , Glicosilfosfatidilinositóis/imunologia , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/imunologia , Mycobacterium leprae/genética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/imunologiaRESUMO
The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form