RESUMO
Desoxyfructo-serotonin (DFS) has shown good results in clinical trials of LL patients. After clinical trials in Bamako (Mali) reported in three articles, clinical trials began in India, at Bombay. Acute toxicity tests done in Paris and chronic toxicity tests done in India had shown absence of side effects. This was also confirmed after pre-clinical pharmacology. In vitro tests show that DFS enhances cellular immune response. Receptors for anti-erythrocyte antibody on LL macrophages are demonstrated by erythrocyte rosetting. Infection with M. leprae markedly reduces rosetting. But in the presence of DFS this reduction in rosetting is not observed. Patient's peripheral blood lymphocytes, sensitised with leprosy antigen, show a low level of rosetting with patients' macrophages. DFS greatly enhances the lymphocyte-macrophage interaction. DFS has an important anti-stress activity. Gastric ulcer induced in rats by restraint were reduced by 40% (Mester et al.) and 50% (Das Neves). DFS increased the uptake of serotonin by LL patients platelets. HPLC studies were done to see the level of DFS in the plasma, in the serum and in the urine of LL patients and controls. We are synthetising new lyposoluble derivatives in order to make easier the penetration of DFS and a long time effect.
Assuntos
Hansenostáticos/uso terapêutico , Hanseníase Virchowiana/tratamento farmacológico , Serotonina/análogos & derivados , Ensaios Clínicos como Assunto , Humanos , Hansenostáticos/farmacocinética , Hanseníase Virchowiana/imunologia , Hanseníase Virchowiana/metabolismo , Formação de Roseta , Serotonina/farmacocinética , Serotonina/uso terapêuticoRESUMO
To examine the anti-leprosy effect of deoxyfructoserotonin the drug was given in a dose of 10 mg/kg for 6 months to 6 patients with active lepromatous leprosy, in accordance with the WHO-THELEP protocol. Clinical and histological assessment and mouse foot-pad studies suggest that the drug has some anti-leprosy effect.
Assuntos
Hansenostáticos/uso terapêutico , Hanseníase/tratamento farmacológico , Serotonina/análogos & derivados , Adulto , Biópsia , Cápsulas , Ensaios Clínicos como Assunto , Humanos , Isoniazida/administração & dosagem , Isoniazida/urina , Hansenostáticos/administração & dosagem , Hanseníase/patologia , Masculino , Serotonina/administração & dosagem , Serotonina/uso terapêutico , Fatores de TempoAssuntos
Hanseníase/imunologia , Mycobacterium leprae/efeitos dos fármacos , Serotonina/análogos & derivados , Animais , Células Cultivadas , Humanos , Imunidade Celular , Hanseníase/microbiologia , Hanseníase/patologia , Macrófagos/efeitos dos fármacos , Macrófagos/microbiologia , Camundongos , Células de Schwann/efeitos dos fármacos , Células de Schwann/microbiologia , Nervo Isquiático/efeitos dos fármacos , Serotonina/farmacologiaRESUMO
Mycobacterium leprae separated from armadillo tissues stored at -80 degrees C is similar to that from human sources in its ability to take up 3H-labelled 3,4-dihydroxyphenylalanine (DOPA). Several inhibitors were studied which showed complete or partial inhibition of [3H]DOPA uptake. These findings suggest that M. leprae isolated from frozen tissue possesses an active uptake system for [3H]DOPA.
Assuntos
Tatus/microbiologia , Di-Hidroxifenilalanina/metabolismo , Mycobacterium leprae/metabolismo , Xenarthra/microbiologia , Animais , Ácido Ascórbico/farmacologia , Quelantes/farmacologia , Baço/microbiologia , TemperaturaRESUMO
Mycobacterium leprae separated from armadillo tissues stored at -80 degrees C is similar to that from human sources in its ability to take up 3H-labelled 3,4-dihydroxyphenylalanine (DOPA). Several inhibitors were studied which showed complete or partial inhibition of [3H]DOPA uptake. These findings suggest that M. leprae isolated from frozen tissue possesses an active uptake system for [3H]DOPA.