RESUMO
Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and of 28 tuberculoid leprosy patients reacted in a significant manner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy patients, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguishing two polar types of leprosy and for diagnosing leprosy in general.
Assuntos
Anticorpos Antibacterianos/análise , Ensaio de Imunoadsorção Enzimática , Hanseníase/imunologia , Mycobacterium tuberculosis/imunologia , Antígenos de Bactérias , Diagnóstico Diferencial , Humanos , Imunoglobulina G/análise , Hanseníase/diagnóstico , Hanseníase Virchowiana/diagnóstico , Hanseníase Virchowiana/imunologia , Hanseníase Tuberculoide/diagnóstico , Hanseníase Tuberculoide/imunologia , Peso MolecularRESUMO
Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and 28 tuberculoid leprosy patient reacted in a significant amnner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy pateints, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguihing two polar types of leprosy and for diagnosing leprosy in general