RESUMO
We report a case of peripheral neuropathy following highly active antiretroviral therapy (HAART) initiation in a patient coinfected with HIV and Mycobacterium leprae and review the literature on leprosy-associated immune reconstitution inflammatory syndrome (IRIS). Physicians in charge of HIV-infected patients originating from countries endemic for leprosy should be aware of this risk of leprosy-associated IRIS when starting HAART.
Assuntos
Terapia Antirretroviral de Alta Atividade/estatística & dados numéricos , Infecções por HIV/complicações , Infecções por HIV/tratamento farmacológico , Hanseníase/complicações , Doenças do Sistema Nervoso Periférico/etiologia , Infecções Oportunistas Relacionadas com a AIDS/complicações , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Adulto , Infecções por HIV/virologia , Humanos , Síndrome Inflamatória da Reconstituição Imune/etiologia , Hanseníase/microbiologia , Masculino , Mycobacterium leprae , Doenças do Sistema Nervoso Periférico/fisiopatologia , Índice de Gravidade de DoençaRESUMO
Leprosy, a chronic human disease with potentially debilitating neurological consequences, results from infection with Mycobacterium leprae. This unculturable pathogen has undergone extensive reductive evolution, with half of its genome now occupied by pseudogenes. Using comparative genomics, we demonstrated that all extant cases of leprosy are attributable to a single clone whose dissemination worldwide can be retraced from analysis of very rare single-nucleotide polymorphisms. The disease seems to have originated in Eastern Africa or the Near East and spread with successive human migrations. Europeans or North Africans introduced leprosy into West Africa and the Americas within the past 500 years.
Assuntos
Emigração e Imigração , Hanseníase/história , Mycobacterium leprae/genética , África/epidemiologia , América/epidemiologia , Ásia/epidemiologia , Evolução Biológica , Europa (Continente)/epidemiologia , Genes Bacterianos , Genoma Bacteriano , História do Século XVIII , História do Século XIX , História Antiga , História Medieval , Humanos , Sequências Repetitivas Dispersas , Hanseníase/epidemiologia , Hanseníase/microbiologia , Hanseníase/transmissão , Repetições Minissatélites , Mycobacterium leprae/classificação , Análise de Sequência com Séries de Oligonucleotídeos , Polimorfismo de Nucleotídeo Único , Dinâmica Populacional , Pseudogenes , Análise de Sequência de DNARESUMO
Leprosy, a chronic human disease with potentially debilitating neurological consequences, results from infection with Mycobacterium leprae. This unculturable pathogen has undergone extensive reductive evolution, with half of its genome now occupied by pseudogenes. Using comparative genomics, we demonstrated that all extant cases of leprosy are attributable to a single clone whose dissemination worldwide can be retraced from analysis of very rare single-nucleotide polymorphisms. The disease seems to have originated in Eastern Africa or the Near East and spread with successive human migrations. Europeans or North Africans introduced leprosy into West Africa and the Americas within the past 500 years.
Assuntos
Humanos , História Antiga , História Medieval , História do Século XVIII , História do Século XIX , Ásia/epidemiologia , América/epidemiologia , Pseudogenes , Genoma Bacteriano , Análise de Sequência com Séries de Oligonucleotídeos , Polimorfismo de Nucleotídeo Único , África/epidemiologia , Emigração e Imigração , Europa (Continente)/epidemiologia , Genes Bacterianos , Hanseníase/história , Hanseníase/microbiologia , Hanseníase/transmissão , Hanseníase/epidemiologia , Mycobacterium leprae/classificação , Mycobacterium leprae/genéticaRESUMO
Erythema nodosum leprosum (ENL) is a well-known serious complication affecting 10% of lepromatous multibacillary leprosy patients. In the chronic form, its morbidity may be considerable. Thalidomide and systemic steroids are the two current effective drugs for the management of ENL. However, their use in endemic countries is often difficult and hazardous, and a search for new therapies is needed. We report our experience on the effects of pentoxifylline, a methylxanthine derivative, which has recently been suggested as a possible effective treatment for ENL attacks.
Assuntos
Eritema Nodoso/tratamento farmacológico , Hansenostáticos/uso terapêutico , Hanseníase Virchowiana/tratamento farmacológico , Pentoxifilina/uso terapêutico , Administração Oral , Adolescente , Adulto , Esquema de Medicação , Eritema Nodoso/patologia , Feminino , Humanos , Hansenostáticos/administração & dosagem , Hanseníase Virchowiana/patologia , Masculino , Pentoxifilina/administração & dosagem , Índice de Gravidade de Doença , Resultado do TratamentoRESUMO
Erythema nodosum leprosum (ENL) is a well-known serious complication affecting 10 per cent of lepromatous multibacillary leprosy patients. In the chronic form, its morbidity may be considerable. Thalidomide and systemic steroids are the two current effective drugs for the management of ENL. However, their use in endemic countries is often difficult and hazardous, and a search for new therapies is needed. We report our experience on the effects of pentoxifylline, a methylxanthine derivative, which has recently been suggested as a possible effective treatment for ENL attacks.
Assuntos
Masculino , Feminino , Humanos , Adulto , Adolescente , Administração Oral , Eritema Nodoso/patologia , Eritema Nodoso/tratamento farmacológico , Esquema de Medicação , Hansenostáticos/administração & dosagem , Hansenostáticos/uso terapêutico , Hanseníase Virchowiana/patologia , Hanseníase Virchowiana/tratamento farmacológico , Pentoxifilina/administração & dosagem , Pentoxifilina/uso terapêutico , Resultado do Tratamento , Índice de Gravidade de DoençaRESUMO
Granuloma annulare is a common granulomatous infiltration of the skin of unknown etiopathogenesis. We analyzed granuloma annulare biopsies in 11 patients and could find in all patients significant numbers of CD4-T cells. These cells showed a broad usage of the different T cell receptor Vbeta families and a rather unbiased repertoire when the complementary determining region 3 spectra were analyzed by the Immunoscope technique. Comparison with the peripheral blood mononuclear cell repertoire, however, identified in all patients few skin-specific expansions, which were for one patient also present in two distinct skin sites. Extensive sequence analysis of the complementary determining region 3 region confirmed the presence of a limited number of skin-specific expansions together with various nonspecific T cell infiltrations. Analysis of the intralesional cytokine expression revealed abundant production of interleukin-2, which was not dominant in granulomas from leprosy patients and was not reflected by the cytokine profile in peripheral blood mononuclear cells. These results demonstrate the capacity of the granulomatous response to recruit T cells in high numbers with only few clones expanding specifically. The high local production of interleukin-2 might thereby play an important role in the nonspecific attraction of T cells to the granulomatous site.
Assuntos
Linfócitos T CD4-Positivos/imunologia , Granuloma Anular/imunologia , Interleucina-2/imunologia , Adulto , Idoso , Linfócitos T CD4-Positivos/química , Linfócitos T CD4-Positivos/citologia , Regiões Determinantes de Complementaridade/genética , Regiões Determinantes de Complementaridade/imunologia , Citocinas/genética , Citocinas/imunologia , Feminino , Expressão Gênica/imunologia , Humanos , Imuno-Histoquímica , Interleucina-2/genética , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Receptores de Antígenos de Linfócitos T alfa-beta/análise , Receptores de Antígenos de Linfócitos T alfa-beta/genética , Receptores de Antígenos de Linfócitos T alfa-beta/imunologia , Análise de Sequência de DNA , Transcrição Gênica/imunologiaRESUMO
The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5 percent gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals
Assuntos
Humanos , Feminino , Adolescente , Idoso , Pessoa de Meia-Idade , Adulto , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/imunologia , Coleta de Amostras Sanguíneas/métodos , Glicolipídeos/sangue , Hanseníase/imunologia , Mycobacterium leprae/imunologia , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/sangue , Ensaio de Imunoadsorção Enzimática , Hanseníase/sangueAssuntos
Anticorpos Antibacterianos/imunologia , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/sangue , Coleta de Amostras Sanguíneas/métodos , Ensaio de Imunoadsorção Enzimática , Glicolipídeos/sangue , Hanseníase/imunologia , Hanseníase/sangue , Mycobacterium leprae/imunologiaRESUMO
Between 1980 and 1994, 67 new or relapsing leprosy patients were treated by daily administered multidrug regimens. Tuberculoid patients (23 TT/BT) received either bitherapy [rifampin + dapsone or clofazimine (RMP + DDS or CLO)] or tritherapy [RMP + DDS and/or CLO and/or ethionamide (ETH)] until clinical cure. Lepromatous patients (44 BB/BL/LL) received tritherapy (RMP + DDS and/or CLO and/or ETH) at least until bacteriological negativity. Of the 23 tuberculoid patients only one patient (5%) was cured at 6 months and about 70% needed between 6 and 24 months of treatment to obtain clinical cure (mean 19.5 months). In the 44 lepromatous patients, the achievement of bacteriological negativity was significantly linked to the initial bacterial index (BI), and it occurred after 2 to 7 years (mean 66.5 months) of multidrug therapy (MDT). The average BI decrease per year was 1.1+ during the first year, 0.9+ the second year, and then < 0.5+ per year. Reactional states significantly (p < 0.01) influenced the BI course: reversal reactions (RR) accelerated while erythema nodosum leprosum (ENL) delayed the BI decrease. Three of the 23 (13%) tuberculoid and 19 of the 44 (43%) lepromatous patients (p < 0.02) exhibited a RR and 18 of 44 (41%) lepromatous patients had ENL during MDT. A late RR (LRR) was observed in 1 (5%) and 6 (17%) of our tuberculoid and lepromatous patients, respectively, and 3 (8%) of our lepromatous patients suffered post-MDT ENL. No confirmed relapse has been observed within a follow-up period of 6 months to 7 years and 3 months [59 person-years at risk (PYR)] for TT/BT patients and of 4 months to 5 years and 10 months (100 PYR) for BB/BL/LL patients. When compared to the recommended WHO/MDT, it appears that daily MDT does not increase the clinical or the bacteriological cure rates either at 6 months in paucibacillary tuberculoid patients or at 2d years in multibacillary lepromatous patients. Moreover, as does the WHO/MDT, our regimens show a high frequency of reactional states both during and after treatment. This fact constitutes the main new problem of the actual treatment of leprosy.
Assuntos
Masculino , Feminino , Humanos , Hanseníase Dimorfa/tratamento farmacológico , Hanseníase Tuberculoide/tratamento farmacológico , Hanseníase Virchowiana/tratamento farmacológicoRESUMO
Among 39 strains of Mycobacterium leprae isolated from patients with multibacillary leprosy who relapsed after treatment with rifampin (RMP), 22 strains were resistant to RMP and 17 were susceptible. All of the RMP-resistant strains were recovered from patients who had been treated with more than 50 doses of RMP, usually given as monotherapy. RMP-susceptible strains were recovered from only six patients who had received more than 50 doses of RMP, and from 11 patients who had received no more than seven doses. The median time to relapse after the beginning of RMP therapy was 9 years (range 1-12 years) among the patients harboring RMP-resistant strains of M. leprae, and the median time to relapse after discontinuation of RMP treatment was 7 years (range 1-11 years) among the patients harboring RMP-susceptible strains. These data suggest that monotherapy with more than a few doses of RMP can be responsible for the emergence of RMP-resistant strains of M. leprae, thus emphasizing the need to employ RMP only in combination with other effective drugs in the chemotherapy of multibacillary leprosy