RESUMO
A taxa de transformação blástica dos linfócitos induzida pela fitohemaglutinina, bem como os níveis de globulinas séricas foram investigados em soldados brasileiros caucasóides que apresentavam reação tardia à lepromina (reação de Mitsuda) fortemente positiva ou completamente negativa. Nenhum dos indivíduos examinados era comunicante de pacientes com hanseníase. Os resultados obtidos permitem concluir que, em indivíduos sadios, nem a transformação dos linfócitos estimulada pela fitohemaglutinina, nem os níveis de globulinas séricas dependem da reação de Mitsuda. A correlação negativa significativa entre o nível de globulinas não gama e a ataxa de transformação blástica dos linfócitos provocada pela fitohemaglutinina, que é encontrada em pacientes lepromatosos, não foi observada nos indivíduos sadios que foram examinados.
Assuntos
Hanseníase/imunologia , Antígeno de Mitsuda , Fito-Hemaglutininas , Globulinas , LinfócitosRESUMO
Chromosome analyses were made on leukocyte metaphases of 18 leprosy patients who were ingesting daily doses of 50 mg or 100 mg of DDS and of 40 healthy individuals used for control. These analyses have shown that the proportion of numerical chromosomal aberrations in the leukocyte metaphases of the leprosy patients did not differ significantly from that observed in the cells of the controls. In contrast, the frequency of cells with chromatid or chromosome breaks and gaps was significantly increased in the leukocytesof leprosy patients. Multiple regression analysis applied to the data recorded has shown that the increase of breaks and gaps in the chromosomes of leprosy patients can not be attributed to age, years under sulfonetherapy or to concentration of DDS in blood.
Assuntos
Hanseníase/genética , Hanseníase/terapia , Hanseníase/tratamento farmacológico , Aberrações Cromossômicas/genética , Sulfonas/administração & dosagem , Sulfonas/farmacocinética , Sulfonas/farmacologia , Sulfonas/química , Sulfonas/sangue , Sulfonas/toxicidade , Sulfonas/uso terapêuticoRESUMO
The proportion of accumulated metaphases, as well as the frequency of aneuploidies and structural chromosome aberrations were investigated in DDS-treated and untreated (controls) leukocyte cultures from two samples of healthy adult Caucasoid individuals. In one sample, the sulfone-treated cultures differed from the controls in that they contained 0.4 mug of DDS per ml of tissue culture medium plus phytohemagglutinin. In the other sample, these cultures differed from the controls in that they contained a tenfold higher concentration of DDS. The two concentrations of DDS used have not significantly affected the rate of metaphases, while 0.4 mug/ml DDS did not increase the frequency of chromosome aberrations. The same was not true for the cultures treated with 4 mug/ml DDS in which the proportion of aneuploides and achromatic gaps increased significantly.