RESUMO
No disponible
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Conferências de Saúde/organização & administração , Astenia/complicações , Astenia/tratamento farmacológico , Exantema/complicações , Derrame Pleural/complicações , Ferro/uso terapêutico , Síndromes de Malabsorção/complicações , Síndromes de Malabsorção/diagnóstico , Linfoma/complicações , Doença de Whipple/complicações , Radiografia Torácica/métodos , Gastroscopia/instrumentação , Redução de Peso , Gastroscopia/métodos , Doença de Whipple/diagnóstico , Colonoscopia/métodos , Intestino Delgado/patologia , Intestino Delgado , Pericardite Constritiva/complicações , Pericardite Constritiva/diagnósticoRESUMO
The immunodeficiency present in patients with lepromatous leprosy is characterized by the limited proliferation of T lymphocytes, and is explained in part by the impaired synthesis of interleukin-2 (IL-2). Diacylglycerol (DAG) and calcium produce the activation of PKC, ERK and JNK kinases, implying a normal IL-2 response. Phorbol esters, such as PMA, can substitute for DAG and are mitogenic to human T and B cells activating several cytokine-encoding genes. Ionophore A23187 increases calcium permeability across the cellular membrane to the cytosol of lymphoid cells and is considered a co-mitogen of T lymphocytes. Here we report that: 1) PHA-activated T lymphocytes from LL patients can be separated in vitro into two groups: a) responders (R) with a stimulation index (SI) of > 10 and (b) nonresponders (NR) with a SI of < 10. 2) The proliferative responses of cells from LL(R), LL(NR) and normal subjects were measured after being stimulated with: I, PHA, PMA, PMA + I PHA + PMA and PHA + PMA + ionophore (PPI). The most important result occurs in LL(NR) patients whose cells did not respond to PHA stimulation but increased to normal levels of proliferation when they were stimulated with PMA. Furthermore, the three groups, (NR, R and normals) strongly increased their responses when they were incubated with PPi. 3) Finally, Il-2 concentrations in the supernatants of cultures of T lymphocytes from LL(NR), LL(R) and controls were relatively low when they were incubated with PHA or PMA, but the addition of ionophore to PMA and the combination of PHA + PMA strongly increased the production of IL-2 in all of them, reaching the optimum IL-2 concentration when PPI is used. It can be concluded that the use of PMA, analogous to DAG, and ionophore A23187 (calcium increaser) in cultures of mitogen-activated T lymphocytes from LL patients induced the expression of the IL-2 gene, thus correcting the inadequate proliferation of T cells from LL patients.
Assuntos
Calcimicina/farmacologia , Divisão Celular/efeitos dos fármacos , Interleucina-2/metabolismo , Hanseníase Virchowiana/imunologia , Linfócitos T/imunologia , Acetato de Tetradecanoilforbol/farmacologia , Adulto , Idoso , Células Cultivadas , Feminino , Humanos , Ativação Linfocitária/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Fito-Hemaglutininas/farmacologiaRESUMO
A great diversity of antigens from Mycobacterium leprae have been described. One practical approach should be to utilize them as markers to indicate when a household contact is at risk of becoming infected and then moving to an active form of leprosy. For this purpose, sonic extracts of M. leprae were fractionated in 10% SDS-PAGE under reducing conditions. The fractionated proteins were then transferred to nitrocellulose sheets and incubated with sera from lepromatous leprosy cases, their contacts, and normal subjects in order to reveal the frequency of antigen recognition of each set of sera. The results showed that sera from lepromatous leprosy patients frequently recognized two proteins, one of approximately 28 kDa and the other of approximately 65 kDa, when compared with the sera from normal subjects. The contacts frequently recognized an approximately 16-kDa antigenic band, while sera from normal subjects recognized one protein of approximately 18 kDa. According to the results, the four recognized proteins from M. leprae can be considered markers of the above conditions (approximately 65 kDa, approximately 28 kDa for lepromatous leprosy, approximately 16 kDa for contacts, and approximately 19 kDa for normal subjects). From these, an easy serological test, such as an ELISA, can be developed to predict if a contact is moving toward lepromatous leprosy before detection of the actual clinical signs or symptoms.
Assuntos
Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/imunologia , Hanseníase Virchowiana/imunologia , Mycobacterium leprae/imunologia , Adulto , Idoso , Reações Antígeno-Anticorpo , Western Blotting , Busca de Comunicante , Eletroforese em Gel de Poliacrilamida , Epitopos/imunologia , Feminino , Humanos , Imunoglobulina G/imunologia , Masculino , Pessoa de Meia-Idade , Peso MolecularRESUMO
Se reporta la correspondente encontrada entre los signos, síntomas y los niveles de los inmunocomplejos circulantes, los que van disminuyendo a medida que la evolucón del pacientes se hace más favorable, se discuten los resultados y de su análisis se sugiere la continuación de nuevas investigaciones en este campo.