Exploring the Efficacy and Mechanisms of a Crop Sterilant for Reducing Infestation by Spotted-Wing Drosophila (Diptera: Drosophilidae).

Vinegar flies (Diptera: Drosophilidae) are well known to be associated with yeasts, which provide important nutrients and emit attractive semiochemicals. Drosophila suzukii (Matsumura) has become a major pest of berries and cherries around the world, requiring intensive management to maintain fruit quality. Although insecticides remain a dominant control approach, disruption of fly-yeast-host interactions remains a promising avenue for reducing the economic impact of this pest. We conducted field and laboratory experiments to explore whether a crop sterilant (peroxyacetic acid and hydrogen peroxide) developed for disease control can affect D. suzukii. In 2 yr of field tests in highbush blueberries, we found significantly lower infestation by D. suzukii in plots treated with the crop sterilant, both alone and in a rotation program with zeta-cypermethrin. When shoots from treated plots were tested in no-choice bioassays, crop sterilant treatments did not affect adult mortality or oviposition, but they reduced infestation. To explore the mechanisms in the laboratory, we found that the crop sterilant did not affect adult mortality, nor oviposition on treated fruit under no-choice settings, but adult flies settled and oviposited less on treated fruit in choice settings. When the crop sterilant was applied to colonies of Hanseniaspora uvarum (Niehaus) (Saccharomycetales: Saccharomycodaceae) and Issatchenkia terricola (Van der Walt) (Saccharomycetales: Saccharomycetacea) yeasts that are attractive and provide nutrition to D. suzukii, there was a dose-dependent inhibition of their growth. We highlight the potential for microbial management as a component of integrated pest management programs and prioritize research needs to incorporate this approach into control programs.

Laboratory and Field Evaluation of Host-Related Foraging Odor-Cue Combinations to Attract Drosophila suzukii (Diptera: Drosophilidae).

The invasive spotted-wing drosophila, Drosophila suzukii (Matsumura), is a major pest of soft-skinned fruits. Since its introduction into North America and Europe, significant progress has been made in understanding the volatile cues used by this fly during food, oviposition site, and mate finding. Despite this progress, commercially available lures are non-selective. Here, we tested two Hanseniaspora uvarum (Niehaus) yeast compounds (isoamyl acetate and isobutyl acetate) and a leaf compound ß-cyclocitral alone and in combination with a blend of four fermentation compounds ('Fermentation lure': acetic acid, ethanol, methionol, and acetoin) to improve D. suzukii attraction and selectivity. In laboratory assays, males and females were attracted to all seven individual compounds, although in electrophysiological assays, their antennae exhibited a dose-dependent response to only four of these compounds. In two-choice cage studies, the Fermentation lure was more attractive to D. suzukii than water controls, whereas ß-cyclocitral and the mixture of isoamyl acetate and isobutyl acetate were not attractive in this larger-cage study. Moreover, adding the two-component H. uvarum compound blend to the Fermentation lure reduced D. suzukii attraction to the Fermentation blend. When these experiments were repeated in blueberry, raspberry, blackberry, and cherry orchards across several states in the United States over 2 yr, similar outcomes were observed: ß-cyclocitral or the mixture of the H. uvarum blend did not improve the attractiveness of the Fermentation lure or its selectivity. This study demonstrates that cues from different sources may interfere with each other and reduce D. suzukii attraction to otherwise attractive odor combinations.