RESUMO
Postharvest losses of fruits and vegetables can reach up to 25% in developed and up to 50% in developing countries. (Sub)tropical fruits are especially susceptible because their protecting peel can easily be damaged. Traditionally used pesticides are associated to environmental pollution and possible harmful health effects. An alternative are biocontrol agents (BCA), means bacteria or yeasts applied onto the fruits to inhibit the growth of phytopathogens. Many reports on their effectiveness have been published, however, reports on their harmlessness to consumers are still rare. Culture extracts of six BCAs, tested on two human lines (Caco-2, HeLa), exhibited no cytotoxic effect, when used directly (1×) to protect the fruits; however, when they are 5×overconcentrated, the confluence of proliferating cells was reduced, but not of differentiated Caco-2. In both cases necrosis was not increased. On proliferating cells, the 5×-extract from Cryptococcus laurentii or Debaryomyces hansenii reduced lysosome functionality and the 6.25×extract from Meyerozyma guilliermondii or Candida famata increased membrane permeability, while only the 25×-extract from M. guilliermondii or M. caribbica reduced slightly the metabolic activity. The extract of Bacillus subtilis showed no cytotoxic effect up to 10× concentration. Overall, their low cytotoxicity combined with high biodegradability make these products suitable for sustainable agriculture.
Assuntos
Bacillus subtilis/química , Agentes de Controle Biológico/efeitos adversos , Conservantes de Alimentos/efeitos adversos , Frutas/microbiologia , Leveduras/química , Bacillus subtilis/metabolismo , Agentes de Controle Biológico/análise , Agentes de Controle Biológico/metabolismo , Células CACO-2 , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Qualidade de Produtos para o Consumidor , Conservação de Alimentos , Conservantes de Alimentos/análise , Conservantes de Alimentos/metabolismo , Células HeLa , Humanos , Leveduras/metabolismoRESUMO
A total of 15 Debaryomyces hansenii strains from different food origins were genetically characterized and tested on a culture medium resembling the composition of fermented sausages but different concentrations of nitrifying preservatives. Genetic typing of the D. hansenii strains revealed two levels of discrimination: isolation source or strain specific. Different abilities to proliferate on culture media containing different concentrations of nitrate and nitrite, as sole nitrogen sources and in the presence of amino acids, were observed within D. hansenii strains. Overall metabolism of amino acids and generation of aroma compounds were related to the strain origin of isolation. The best producers of branched aldehydes and ethyl ester compounds were strains isolated from pork sausages. Strains from cheese and llama sausages were good producers of ester compounds and branched alcohols, while vegetable strains produced mainly acid compounds. Nitrate and nitrite reduction affected in different ways the production of volatiles by D. hansenii.