Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros


Bases de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Food Microbiol ; 94: 103670, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33279092

RESUMO

The use of non-Saccharomyces species as starter cultures together with Saccharomyces cerevisiae is becoming a common practice in the oenological industry to produce wines that respond to new market demands. In this context, microbial interactions with these non-Saccharomyces species must be considered for a rational design of yeast starter combinations. Previously, transcriptional responses of S. cerevisiae to short-term co-cultivation with Torulaspora delbrueckii, Candida sake, or Hanseniaspora uvarum was compared. An activation of sugar consumption and glycolysis, membrane and cell wall biogenesis, and nitrogen utilization was observed, suggesting a metabolic boost of S. cerevisiae in response to competing yeasts. In the present study, the transcription profile of S. cerevisiae was analyzed after 3 h of cell contact with Metschnikowia pulcherrima. Results show an over-expression of the gluco-fermentative pathway much stronger than with the other species. Moreover, a great repression of the respiration pathway has been found in response to Metschnikowia. Our hypothesis is that there is a direct interaction stress response (DISR) between S. cerevisiae and the other yeast species that, under excess sugar conditions, induces transcription of the hexose transporters, triggering glucose flow to fermentation and inhibiting respiration, leading to an increase in both, metabolic flow and population dynamics.


Assuntos
Metschnikowia/metabolismo , Saccharomyces cerevisiae/metabolismo , Aerobiose , Parede Celular/genética , Parede Celular/metabolismo , Técnicas de Cocultura , Fermentação , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Glicólise , Metschnikowia/genética , Metschnikowia/crescimento & desenvolvimento , Oxigênio/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Vinho/análise
2.
J Sci Food Agric ; 97(11): 3594-3602, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28098345

RESUMO

BACKGROUND: There has been an increasing interest in the use of selected non-Saccharomyces yeasts in co-culture with Saccharomyces cerevisiae. In this work, three non-Saccharomyces yeast strains (Metschnikowia viticola, Metschnikowia fructicola and Hanseniaspora uvarum) indigenously isolated in Denmark were used in sequential fermentations with S. cerevisiae on three cool-climate grape cultivars, Bolero, Rondo and Regent. During the fermentations, the yeast growth was determined as well as key oenological parameters, volatile compounds and sensory properties of finished rosé wines. RESULTS: The different non-Saccharomyces strains and cool-climate grape cultivars produced wines with a distinctive aromatic profile. A total of 67 volatile compounds were identified, including 43 esters, 14 alcohols, five acids, two ketones, a C13-norisoprenoid, a lactone and a sulfur compound. The use of M. viticola in sequential fermentation with S. cerevisiae resulted in richer berry and fruity flavours in wines. The sensory plot showed a more clear separation among wine samples by grape cultivars compared with yeast strains. CONCLUSION: Knowledge on the influence of indigenous non-Saccharomyces strains and grape cultivars on the flavour generation contributed to producing diverse wines in cool-climate wine regions. © 2017 Society of Chemical Industry.


Assuntos
Aromatizantes/química , Hanseniaspora/metabolismo , Metschnikowia/metabolismo , Saccharomyces cerevisiae/metabolismo , Vitis/química , Compostos Orgânicos Voláteis/química , Vinho/análise , Adulto , Dinamarca , Feminino , Fermentação , Aromatizantes/metabolismo , Hanseniaspora/crescimento & desenvolvimento , Humanos , Masculino , Metschnikowia/crescimento & desenvolvimento , Saccharomyces cerevisiae/crescimento & desenvolvimento , Paladar , Vitis/metabolismo , Vitis/microbiologia , Compostos Orgânicos Voláteis/metabolismo , Vinho/microbiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA