RESUMO
TLRs are thought to play a role in the pathophysiology of such dermatological diseases as leprosy, acne and psoriasis. The study included 20 patients with plaque psoriasis, as well as 20 healthy age- and gender-matched control subjects. Real-time polymerase chain reaction evaluation was made of the messenger RNA expression of TLRs 1-10 in lesional tissue and peripheral blood mononuclear cell samples in psoriasis patients. TLR 3, 5, 6, 7, 9 and 10 lesional tissue mRNA expressions were increased significantly when compared to the expression levels in the PBMCs of the same patients (pâ¯=â¯0.0082, pâ¯=â¯0.0176, pâ¯=â¯0.0239, pâ¯=â¯0.0261, pâ¯=â¯0.0223, pâ¯=â¯0.0206). A comparison of the TLR expression in the PBMCs of healthy subjects and the PBMCs of patients with psoriasis showed a significant increase in the TLR 1, 8 and 10 mRNA expressions in the patient group (pâ¯<â¯0.0001, pâ¯<â¯0.0001, pâ¯=â¯0.0035). The TLR 5 mRNA expression was significantly higher in the control group than in the patient group (pâ¯=â¯0.0037). To the best of our knowledge, this is the first study in literature to evaluate mRNA TLR expression levels in the lesional tissue and PBMCs of patients with psoriasis.