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1.
Biochim Biophys Acta ; 1861(1): 60-67, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26515252

RESUMO

Diseases including tuberculosis and leprosy are caused by species of the Mycobacterium genus and are a huge burden on global health, aggravated by the emergence of drug resistant strains. Mycobacteria have a high lipid content and complex lipid profile including several unique classes of lipid. Recent years have seen a growth in research focused on lipid structures, metabolism and biological functions driven by advances in mass spectrometry techniques and instrumentation, particularly the use of electrospray ionization. Here we review the contributions of lipidomics towards the advancement of our knowledge of lipid metabolism in mycobacterial species.


Assuntos
Metabolismo dos Lipídeos , Mycobacterium/metabolismo , Biologia Computacional , Glicolipídeos/metabolismo , Lipídeos/biossíntese , Espectrometria de Massas , Ácidos Micólicos/metabolismo , Triglicerídeos/metabolismo
2.
Prep Biochem Biotechnol ; 40(1): 28-37, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20024792

RESUMO

A strain isolated from waste of a milk products plant and exhibited extracellular lipolytic activity was identified as Debaryomyces hansenii by 5.8S rRNA and 28S rRNA gene sequence analyses. Lipolytic activity was assayed spectrophotometrically by using p-nitrophenylpalmitate. Higher specific lipolytic activities were obtained in the presence of tristearin (0.68 U/mg prot), oleic acid (0.56 U/mg prot), and soybean oil (0.36 U/mg prot) than other triglycerides, fatty acids, and vegetable oils considered as carbon sources. Cheese whey appeared to be a good alternative to lipidic substances for lipolytic activity. Among various organic and inorganic nitrogen sources, soy flour was found to attain the lipolytic activity similar to that provided by universal yeast medium components. This work is the first report on the discussion of lipolytic activity enhancement by D. hansenii through modulating the cultivation medium. It also proposes low cost medium nutrients that could be of industrial value and could serve as basal nutrients for further optimization studies on the lipase production by D. hansenii.


Assuntos
Debaryomyces/enzimologia , Microbiologia Industrial/métodos , Lipase/biossíntese , Lipase/metabolismo , Queijo , Debaryomyces/isolamento & purificação , Gorduras Insaturadas na Dieta/metabolismo , Ácidos Graxos/metabolismo , Glicerol/metabolismo , Resíduos Industriais , Compostos de Nitrogênio/metabolismo , Octoxinol/metabolismo , Triglicerídeos/metabolismo
4.
Int J Lepr Other Mycobact Dis ; 65(1): 1-11, 1997 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9207748

RESUMO

The concentrations of serum lipids and tumor necrosis factor (TNF) were measured in leprosy patients across the spectrum of the disease and in erythema nodosum leprosum (ENL) patients at the onset of the reaction and after the reaction had clinically subsided. Lepromatous/borderline lepromatous (LL/BL) patients had significantly higher serum triglyceride and lower HDL-cholesterol levels; there was no such change in the tuberculoid/borderline tuberculoid (TT/BT) patients. The household contacts (HC) of the LL/BL patients also had significantly lower serum HDL levels. ENL patients during the acute phase of the reaction had significantly lower total, LDL-, HDL-cholesterol levels compared to the stable LL/BL patients, and these changes were reversible to pre-ENL levels after the reaction had subsided. Serum TNF levels were significantly higher in household contacts and in LL/BL patients but were not statistically different in TT/BT patients. Serum TNF levels were also significantly higher during the acute phase of ENL, and declined after the clinical remission of the reaction to levels comparable with those of LL/BL patients. There was a significant negative correlation between serum TNF and HDL-cholesterol levels during and after ENL reaction. However, there was no such correlation between TNF and total or LDL-cholesterol levels in ENL patients. Our results suggest that the changes in HDL-cholesterol metabolism are a specific part of the host response to lepromatous leprosy and to the ENL reaction and may be mediated by increased TNF production.


Assuntos
HDL-Colesterol/metabolismo , Eritema Nodoso/metabolismo , Hanseníase Virchowiana/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Adolescente , Adulto , Idoso , HDL-Colesterol/análise , HDL-Colesterol/sangue , LDL-Colesterol/análise , LDL-Colesterol/sangue , LDL-Colesterol/metabolismo , Eritema Nodoso/sangue , Feminino , Humanos , Hanseníase Virchowiana/sangue , Hanseníase Tuberculoide/sangue , Hanseníase Tuberculoide/metabolismo , Masculino , Pessoa de Meia-Idade , Triglicerídeos/análise , Triglicerídeos/sangue , Triglicerídeos/metabolismo , Fator de Necrose Tumoral alfa/análise
5.
Indian J Lepr ; 58(3): 367-72, 1986.
Artigo em Inglês | MEDLINE | ID: mdl-3540141

RESUMO

Presence of lipase, in Mycobacterium leprae obtained from human nodules and infected armadillo tissues, has been detected by demonstrating the ability of the bacteria to hydrolyze tributyrin. This capacity is expressed during incubation of the bacteria with the substrate and needs a source of carbon and other energy metabolites. The activity is blocked by anti M. leprae drug rifampicin. It is concluded that expression of lipase activity is a metabolic event of M. leprae, while they are maintained in an energy providing medium.


Assuntos
Lipase/metabolismo , Mycobacterium leprae/enzimologia , Animais , Tatus/microbiologia , Meios de Cultura , Humanos , Mycobacterium leprae/crescimento & desenvolvimento , Triglicerídeos/metabolismo
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