Study of murine experimental Jorge Lobo's disease by analysis of peritoneal lavage cells and footpad histopathology: early versus chronic lesions
Oxford; s.n; 2015. 9 p. ilus.
Non-conventional
em En
| SES-SP, SESSP-ILSLPROD, SES-SP, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1085511
Biblioteca responsável:
BR191.1
Localização: BR191.1; 9452/s
ABSTRACT
The murine model of Jorge Lobo's disease is characterized by histological alterations similar to those seen in human disease, including a large number of viable fungi. This study evaluated the immune response of mice with early and late macroscopic lesions (5 and 13 months post-inoculation [p.i.], respectively) by the analysis of peritoneal lavage cells and footpad (FP) histology. The FP of mice were inoculated with 1 × 106 fungi (viability index of 41%). At 5 and 13 months p.i., the granuloma mainly consisted of macrophages and multinucleated giant cells, but a larger number of neutrophils was observed at 5 months and lymphocytes at 13 months. The number of fungi in the FP and fungal viability were 1.8 ± 1.1 × 106 fungi/ml and 38.5% at 5 months p.i. and 30.8 ± 11.7 × 106 fungi/ml and 9% at 13 months (P < .05). Higher production of H2O2, O2−, IL-10, and TNF-α were observed at 13 months (P < .05), but there was no significant difference in the production of NO, IL-2, IL-4, IL-12 and IFN-γ. The results showed significant differences between early and late lesions and support the use of BALB/c mice for evaluation of the different phases of infection
Palavras-chave
Texto completo:
1
Tema:
Geral
Bases de dados:
SES-SP
Assunto principal:
Seguimentos
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Citocinas
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Fungos
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Granuloma
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Leucócitos
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Camundongos Endogâmicos BALB C
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Óxido Nítrico
Tipo de estudo:
Observational_studies
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Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Non-conventional