Murine experimental leprosy: evaluation of immune response by analysis of peritoneal lavage cells and footpad histopathology
s.l; s.n; 2019. 13 p.
Non-conventional
em En
| HANSEN, SES-SP, CONASS, HANSENIASE, SESSP-ILSLPROD, SES-SP, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1147217
Biblioteca responsável:
BR191.1
Localização: BR191.1; 9794/S
ABSTRACT
This study evaluated the immune response of nude and BALB/c mice inoculated in the footpads (FP) with Mycobacterium leprae after 3, 5 and 8 months. At each timepoint peritoneal cells, peripheral blood, FP and popliteal lymph nodes (PLN) were collected. Peritoneal cell cultures were performed to measure the H2O2, O2−, NO, IL2, IL4, IL10, IL12, IFNγ and TNF levels. Serum levels of antiPGLI antibodies were also quantified. The results showed that the infection was progressive in nude mice with bacterial multiplication, development of macroscopic lesions in the FP and presence of bacilli in the PLN at 8 months. In BALB/c mice, the infection reached a plateau of bacillary multiplication at 5 months and regressed at 8 months. Histopathological analysis of FP revealed a mononuclear inflammatory infiltrate with a large number of neutrophils at 5 months, with a higher number in nude mice. At 8 months, the number of neutrophils decreased and the infiltrate was predominantly mononuclear in both mouse strains. There was no H2O2, O2−, IL2, IL4, IL10 and IFNγ production in the course of infection in nude mice; however, in BALB/c, O2− and IL12 production was higher at 5 months and NO, IFNγ and TNF production was higher at 8 months when there was a decrease in the number of bacilli. The level of antiPGLI antibodies was higher in BALB/c mice. Thus, nude and BALB/c mice can be used as experimental models for the study of various aspects of leprosy(AU).
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Tema:
Complicacoes
/
Geral
Bases de dados:
HANSEN
/
HANSENIASE
/
SES-SP
Assunto principal:
Hanseníase
/
Mycobacterium leprae
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Non-conventional