Rapid detection of Mycobacterium bovis on its lipid profile by thin layer chromatography.
Vet Microbiol
; 65(2): 145-51, 1999 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-10078598
ABSTRACT
Sixteen Mycobacterium bovis (M. bovis) strains isolated from bovine tissues and one standard reference strain of M. bovis AN5 alongwith other species of mycobacteria for comparison were investigated for the presence of phenolic glycolipid (PGL) and phthiocerol dimycocerosate (PDIM) for rapid identification of M. bovis by thin-layer chromatography (TLC). The study indicated presence of PGL with an Rf value of 0.75 in chloroform-methanol solvent in all 17 M. bovis strains. The dimycocerostate A corresponding to spot A was the major constituent among all the three spots in M. bovis strains. TLC appeared to be a promising alternative to conventional biochemical methods for identification of M. bovis taking into consideration both PGL and PDIM lipids.
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Tema:
Epidemiologia
/
Geral
/
Tratamento_medicamentoso
Bases de dados:
MEDLINE
Assunto principal:
Lipídeos
/
Mycobacterium bovis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
1999
Tipo de documento:
Article