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Whole-genome expression analysis of Mycobacterium leprae and its clinical application.
Akama, Takeshi; Suzuki, Koichi; Tanigawa, Kazunari; Nakamura, Kazuaki; Kawashima, Akira; Wu, Huhehasi; Sue, Mariko; Yoshihara, Aya; Ishido, Yuko; Ishii, Norihisa.
Afiliação
  • Akama T; Leprosy Research Center, National Institute of Infectious Diseases, Tokyo 189-0002, Japan.
Jpn J Infect Dis ; 63(6): 387-92, 2010 Nov.
Article em En | MEDLINE | ID: mdl-21099087
The whole-genome sequence analysis of Mycobacterium leprae, which was completed in 2001, revealed the characteristics of this microbe's genomic structure. Half of the M. leprae genome consists of a limited number of protein-coding genes and the rest comprises non-coding regions and pseudogenes. We performed membrane array and tiling array analyses to analyze the gene-expression profile of the M. leprae genome and found that pseudogenes and non-coding regions were expressed similarly to coding regions at the RNA level. The RNA expressions were confirmed by real-time PCR analysis. Expression of these RNAs in clinical samples showed varying patterns among patients, thus indicating that the analysis of RNA expression patterns, including non-coding regions and pseudogenes, may be useful for understanding the pathological state, prognosis, and assessment of therapeutic progress in leprosy.
Assuntos
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Tema: Complicacoes / Etiologia / Geral Bases de dados: MEDLINE Assunto principal: Genoma Bacteriano / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Hanseníase / Mycobacterium leprae Tipo de estudo: Prognostic_studies Idioma: En Revista: Jpn J Infect Dis Ano de publicação: 2010 Tipo de documento: Article
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Tema: Complicacoes / Etiologia / Geral Bases de dados: MEDLINE Assunto principal: Genoma Bacteriano / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Hanseníase / Mycobacterium leprae Tipo de estudo: Prognostic_studies Idioma: En Revista: Jpn J Infect Dis Ano de publicação: 2010 Tipo de documento: Article