Whole-genome expression analysis of Mycobacterium leprae and its clinical application.
Jpn J Infect Dis
; 63(6): 387-92, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-21099087
The whole-genome sequence analysis of Mycobacterium leprae, which was completed in 2001, revealed the characteristics of this microbe's genomic structure. Half of the M. leprae genome consists of a limited number of protein-coding genes and the rest comprises non-coding regions and pseudogenes. We performed membrane array and tiling array analyses to analyze the gene-expression profile of the M. leprae genome and found that pseudogenes and non-coding regions were expressed similarly to coding regions at the RNA level. The RNA expressions were confirmed by real-time PCR analysis. Expression of these RNAs in clinical samples showed varying patterns among patients, thus indicating that the analysis of RNA expression patterns, including non-coding regions and pseudogenes, may be useful for understanding the pathological state, prognosis, and assessment of therapeutic progress in leprosy.
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Tema:
Complicacoes
/
Etiologia
/
Geral
Bases de dados:
MEDLINE
Assunto principal:
Genoma Bacteriano
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Hanseníase
/
Mycobacterium leprae
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Jpn J Infect Dis
Ano de publicação:
2010
Tipo de documento:
Article