Development and pre-clinical assessment of a 73 kD chimeric fusion protein as a defined sub-unit vaccine for leprosy.
Vaccine
; 31(5): 813-9, 2013 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-23228811
ABSTRACT
Despite the advances toward the elimination of leprosy through widespread provision of multi-drug therapy to registered patients over the last 2 decades, new case detection rates have stabilized and leprosy remains endemic in a number of localized regions. A vaccine could overcome the inherent limitations of the drug treatment program by providing protection in individuals who are not already harboring the Mycobacterium leprae bacilli at the time of administration and effectively interrupt the transmission cycle over a wider timespan. In this report we present data validating the production of 73f, a chimeric fusion protein incorporating the M. leprae antigens ML2028, ML2346 and ML2044. The 73f protein was recognized by IgG in multibacillary (MB) leprosy patient sera and stimulated IFNγ production within whole blood assays of paucibacillary (PB) leprosy patient and healthy household contacts of MB patients (HHC). When formulated with a TLR4L-containing adjuvant (GLA-SE), 73f stimulated a strong and pluripotent Th1 response that inhibited M. leprae-induced inflammation in mice. We are using these data to develop new vaccine initiatives for the continued and long-term control of leprosy.
Texto completo:
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Tema:
Complicacoes
/
Epidemiologia
/
Geral
/
Prevencao_controle
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Transmissao
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Tratamento_medicamentoso
Bases de dados:
MEDLINE
Assunto principal:
Vacinas Bacterianas
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Hanseníase
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Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2013
Tipo de documento:
Article