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Field-friendly serological tests for determination of M. leprae-specific antibodies.
van Hooij, Anouk; Tjon Kon Fat, Elisa M; van den Eeden, Susan J F; Wilson, Louis; Batista da Silva, Moises; Salgado, Claudio G; Spencer, John S; Corstjens, Paul L A M; Geluk, Annemieke.
Afiliação
  • van Hooij A; Dept. of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • Tjon Kon Fat EM; Dept. Molecular Cell Biology and Dept. Reumatology, Leiden University Medical Center, Leiden, The Netherlands.
  • van den Eeden SJF; Dept. of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • Wilson L; Dept. of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • Batista da Silva M; Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil.
  • Salgado CG; Laboratório de Dermato-Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Marituba, Pará, Brazil.
  • Spencer JS; Dept. of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, USA.
  • Corstjens PLAM; Dept. Molecular Cell Biology and Dept. Reumatology, Leiden University Medical Center, Leiden, The Netherlands.
  • Geluk A; Dept. of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. A.geluk@lumc.nl.
Sci Rep ; 7(1): 8868, 2017 08 21.
Article em En | MEDLINE | ID: mdl-28827673
Early detection of leprosy is key to reduce the ongoing transmission. Antibodies directed against M. leprae PGL-I represent a useful biomarker for detecting multibacillary (MB) patients. Since efficient leprosy diagnosis requires field-friendly test conditions, we evaluated two rapid lateral flow assays (LFA) for detection of Mycobacterium leprae-specific antibodies: the visual immunogold OnSite Leprosy Ab Rapid test [Gold-LFA] and the quantitative, luminescent up-converting phosphor anti-PGL-I test [UCP-LFA]. Test performance was assessed in independent cohorts originating from three endemic areas. In the Philippine cohort comprising patients with high bacillary indices (BI; average:4,9), 94%(n = 161) of MB patients were identified by UCP-LFA and 78%(n = 133) by Gold-LFA. In the Bangladeshi cohort, including mainly MB patients with low BI (average:1), 41%(n = 14) and 44%(n = 15) were detected by UCP-LFA and Gold-LFA, respectively. In the third cohort of schoolchildren from a leprosy hyperendemic region in Brazil, both tests detected 28%(n = 17) seropositivity. Both rapid tests corresponded well with BI(p < 0.0001), with a fairly higher sensitivity obtained with the UCP-LFA assay. However, due to the spectral character of leprosy, additional, cellular biomarkers are required to detect patients with low BIs. Therefore, the UCP-LFA platform, which allows multiplexing with differential biomarkers, offers more cutting-edge potential for diagnosis across the whole leprosy spectrum.
Assuntos

Texto completo: 1 Tema: Complicacoes / Geral / Transmissao / Tratamento_medicamentoso Bases de dados: MEDLINE Assunto principal: Testes Sorológicos / Testes Imediatos / Hanseníase / Anticorpos Antibacterianos / Mycobacterium leprae Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Tema: Complicacoes / Geral / Transmissao / Tratamento_medicamentoso Bases de dados: MEDLINE Assunto principal: Testes Sorológicos / Testes Imediatos / Hanseníase / Anticorpos Antibacterianos / Mycobacterium leprae Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article