Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767.
Bio Protoc
; 9(17): e3352, 2019 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-33654853
ABSTRACT
Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological potential in the agrofood sector. However, lack of an efficient transformation system in D. hansenii still direct the complementation based assay in S. cerevisiae mutants for functional analysis of D. hansenii genes. Here, we have described the development of an efficient transformation system for D. hansenii that is based on a histidine auxotrophic recipient strain, DBH9 (generated by UV induced random mutagenesis), and the DhHIS4 gene as the selectable marker. Moreover, the same method has also been employed for gene disruption in D. hansenii by homologous recombination.
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Geral
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MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2019
Tipo de documento:
Article