A Mycobacterium leprae gene encoding a fibronectin binding protein is used for efficient invasion of epithelial cells and Schwann cells.
Infect Immun
; 63(7): 2652-7, 1995 Jul.
Article
em En
| MEDLINE
| ID: mdl-7790081
ABSTRACT
Mycobacterium leprae, the causative agent of leprosy, is an obligate intracellular pathogen. M. leprae can infect a variety of cells in vivo, including epithelial cells, muscle cells, and Schwann cells, in addition to macrophages. The ligand-receptor interactions important in the attachment and ingestion of M. leprae by these nonmacrophage cells remains unknown. Fibronectin (FN) significantly enhances both attachment and ingestion of M. leprae by epithelial and Schwann cell lines. We cloned an M. leprae FN binding protein (FN attachment protein [FAP]) distinct from the 85ABC complex which has been shown previously to bind FN. The FAP open reading frame predicts a protein of 29.5 kDa with a 39-amino-acid signal peptide and was previously described as an antigen in leprosy patients. M. leprae FAP has homologies in M. vaccae, M. avium, and M. tuberculosis, as determined by Southern blotting and direct peptide analysis. Both anti-FAP antibodies and an Escherichia coli-expressed recombinant protein significantly blocked M. leprae attachment and internalization by T-24, an epithelial cell line, and JS1, a Schwann cell line. These data suggest that FN can be a bridging opsonic ligand for attachment of mycobacteria to nonphagocytes and that FAP plays an important role in this process. This may be an important step in the initiation of M. leprae infection in vivo.
Texto completo:
1
Tema:
Geral
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Tratamento_medicamentoso
Bases de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Aderência Bacteriana
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Fibronectinas
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Adesinas Bacterianas
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Genes Bacterianos
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Mycobacterium leprae
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Antígenos de Bactérias
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
1995
Tipo de documento:
Article