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Fractionation of mycobacterial integral membrane proteins by continuous elution SDS-PAGE reveals the immunodominance of low molecular weight subunits for human T cells.
Mehrotra, J; Mittal, A; Dhindsa, M S; Sinha, S.
Afiliação
  • Mehrotra J; Division of Membrane Biology, Central Drug Research Institute, Lucknow, India.
Clin Exp Immunol ; 109(3): 446-50, 1997 Sep.
Article em En | MEDLINE | ID: mdl-9328120
Integral membrane proteins (IMP) represent a serologically distinct class of mycobacterial antigens which are potent stimulators of human T cells (Mehrotra et al., Clin Exp Immunol 1995; 102:626). The range of IMP from Mycobacterium fortuitum was resolved by continuous elution SDS-PAGE to recover 31 discrete fractions covering bands up to approximately 58 kD. The fractions, after removal of SDS, were subjected to human T cell proliferation assays for the identification of immunodominant molecule(s). A low molecular weight (<20kD) fraction was able to stimulate T cells from 11 out of 12 donors comprising mainly tuberculoid leprosy patients. The described protocol is well suited to situations where large quantities of antigenic protein mixtures must be processed in order to get the purified molecules/fractions in amounts required for immunoepidemiological studies.
Assuntos

Texto completo: 1 Tema: Geral / Tratamento_medicamentoso Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Linfócitos T / Proteínas de Membrana / Micobactérias não Tuberculosas Idioma: En Revista: Clin Exp Immunol Ano de publicação: 1997 Tipo de documento: Article

Texto completo: 1 Tema: Geral / Tratamento_medicamentoso Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Linfócitos T / Proteínas de Membrana / Micobactérias não Tuberculosas Idioma: En Revista: Clin Exp Immunol Ano de publicação: 1997 Tipo de documento: Article