ABSTRACT
B cells and the antibodies they produce have a deeply penetrating influence on human physiology. Here, we review current understanding of how B cell responses are initiated; the different paths to generate short- and long-lived plasma cells, germinal center cells, and memory cells; and how each path impacts antibody diversity, selectivity, and affinity. We discuss how basic research is informing efforts to generate vaccines that induce broadly neutralizing antibodies against viral pathogens, revealing the special features associated with allergen-reactive IgE responses and uncovering the antibody-independent mechanisms by which B cells contribute to health and disease.
Subject(s)
B-Lymphocytes/metabolism , Animals , Antibodies, Neutralizing/immunology , Antibodies, Neutralizing/metabolism , Antigens/immunology , B-Lymphocytes/immunology , Germinal Center/immunology , Germinal Center/metabolism , Humans , Immunologic Memory , Plasma Cells/immunology , Plasma Cells/metabolism , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Vaccines/immunologyABSTRACT
Fas is a cell surface death receptor critical for immune regulation. In this issue of Immunity, Butt et al. (2015) show that Fas eliminates B cells that have become uncoupled from positive and negative selection in the germinal center.
Subject(s)
Autoantibodies/immunology , B-Lymphocytes/cytology , Germinal Center/cytology , Germinal Center/immunology , Immunoglobulin E/immunology , fas Receptor/immunology , Animals , HumansABSTRACT
This Brief Review delves into B cell responses in the context of allergy. The primary contribution of B cells to allergy is the production of IgE, the Ab isotype that triggers immediate hypersensitivity reactions through the release of mediators from mast cells and basophils. B cells may also have protective roles in allergy, such as through the production of IgG or as regulatory B cells. In this review, I focus on the basic principles of B cell differentiation and discuss features relevant to allergic immune responses. In particular, I discuss: (1) class-switch recombination; (2) plasma cell differentiation; (3) germinal centers and affinity maturation; and (4) memory B cells and recall responses, with an emphasis on IgE, IgG1, and IgG4. I also consider how B cells may contribute to allergic responses independent of Ab production-for example, by serving as APCs.
Subject(s)
B-Lymphocytes/immunology , Cell Differentiation/immunology , Hypersensitivity, Immediate/immunology , Immunoglobulin Class Switching/immunology , Immunoglobulin E/immunology , B-Lymphocytes, Regulatory/immunology , Basophils/immunology , Germinal Center/immunology , Humans , Hypersensitivity, Immediate/pathology , Immunoglobulin A/immunology , Immunoglobulin G/immunology , Immunologic Memory/immunology , Lymphocyte Activation/immunology , Mast Cells/immunology , Memory B Cells/immunology , Plasma Cells/cytology , Plasma Cells/immunologyABSTRACT
Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.
Subject(s)
Basophils/immunology , Interleukin-4/immunology , Lung/immunology , Strongylida Infections/immunology , Animals , Basophils/metabolism , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , Female , Flow Cytometry , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Helminthiasis, Animal/immunology , Helminthiasis, Animal/metabolism , Helminthiasis, Animal/parasitology , Host-Parasite Interactions/immunology , Humans , Interleukin-13/genetics , Interleukin-13/immunology , Interleukin-13/metabolism , Interleukin-4/genetics , Interleukin-4/metabolism , Liver/immunology , Liver/metabolism , Liver/parasitology , Lung/metabolism , Lung/parasitology , Lung Diseases, Parasitic/immunology , Lung Diseases, Parasitic/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Microscopy, Fluorescence, Multiphoton , Nippostrongylus/immunology , Nippostrongylus/physiology , Schistosoma mansoni/immunology , Schistosoma mansoni/physiology , Strongylida Infections/metabolism , Strongylida Infections/parasitologyABSTRACT
Mice deficient in sphingosine 1-phosphate receptor type 2 (S1P(2)) develop diffuse large B cell lymphoma. However, the role of S1P(2) in normal germinal center (GC) physiology is unknown. Here we show that S1P(2)-deficient GC B cells outgrew their wild-type counterparts in chronically established GCs. We found that antagonism of the kinase Akt mediated by S1P(2) and its downstream mediators Gα(12), Gα(13) and p115RhoGEF regulated cell viability and was required for growth control in chronically proliferating GCs. Moreover, S1P(2) inhibited GC B cell responses to follicular chemoattractants and helped confine cells to the GC. In addition, S1P(2) overexpression promoted the centering of activated B cells in the follicle. We suggest that by inhibiting Akt activation and migration, S1P(2) helps restrict GC B cell survival and localization to an S1P-low niche at the follicle center.
Subject(s)
B-Lymphocytes/immunology , Germinal Center/immunology , Homeostasis/immunology , Receptors, Lysosphingolipid/immunology , Animals , B-Lymphocytes/enzymology , Cell Survival/immunology , GTP-Binding Protein alpha Subunits, G12-G13/immunology , Germinal Center/cytology , Germinal Center/enzymology , Guanine Nucleotide Exchange Factors/immunology , Mice , Mice, Inbred C57BL , Proto-Oncogene Proteins c-akt/immunology , Rho Guanine Nucleotide Exchange FactorsABSTRACT
Germinal center (GC) B cells cycle between the dark zone (DZ) and light zone (LZ) during antibody affinity maturation. Whether this movement is necessary for GC function has not been tested. Here we show that CXCR4-deficient GC B cells, which are restricted to the LZ, are gradually outcompeted by WT cells indicating an essential role for DZ access. Remarkably, the transition between DZ centroblast and LZ centrocyte phenotypes occurred independently of positioning. However, CXCR4-deficient cells carried fewer mutations and were overrepresented in the CD73(+) memory compartment. These findings are consistent with a model where GC B cells change from DZ to LZ phenotype according to a timed cellular program but suggest that spatial separation of DZ cells facilitates more effective rounds of mutation and selection. Finally, we identify a network of DZ CXCL12-expressing reticular cells that likely support DZ functions.
Subject(s)
B-Lymphocytes/cytology , Germinal Center/cytology , Lymphopoiesis/physiology , Animals , Antibody Affinity , Antigens, Differentiation, B-Lymphocyte/metabolism , Cell Cycle , Cell Movement , Chemokine CXCL12/analysis , Clonal Selection, Antigen-Mediated , Germinal Center/ultrastructure , Immunologic Memory , Lymph Nodes/ultrastructure , Mediastinum , Mice , Orthomyxoviridae Infections/immunology , Peyer's Patches/cytology , Phenotype , Plasma Cells/cytology , Radiation Chimera , Receptors, CXCR4/analysis , Receptors, CXCR4/deficiency , Specific Pathogen-Free Organisms , Time FactorsABSTRACT
IgE antibodies may be protective in parasite immunity, but their aberrant production can lead to allergic disease and life-threatening anaphylaxis. Despite the importance of IgE regulation, few studies have directly examined the B cells that express IgE, because these cells are rare and difficult to detect. Here, we describe fluorescent IgE reporter mice and validate a flow cytometry procedure to allow sensitive and specific identification of IgE-expressing B cells in vivo. Similar to IgG1(+) cells, IgE(+) B cells differentiated into germinal center (GC) B cells and plasma cells (PCs) during primary immune responses to a T cell-dependent hapten-protein conjugate and the helminth Nippostrongylus brasiliensis. However, the participation of IgE(+) B cells in GCs was transient. IgE(+) B cells had an atypical propensity to upregulate the transcription factor Blimp-1 and undergo PC differentiation. Most IgE(+) PCs were short lived and showed reduced affinity maturation, revealing intrinsic mechanisms that restrict the IgE antibody response.
Subject(s)
B-Lymphocytes/immunology , Fluorescent Antibody Technique/methods , Immunoglobulin E/immunology , Immunoglobulin E/metabolism , Amino Acid Sequence , Animals , Apoptosis/immunology , B-Lymphocytes/cytology , B-Lymphocytes/metabolism , Base Sequence , Cell Differentiation/immunology , Chimera/immunology , Chimera/metabolism , Female , Germinal Center/cytology , Germinal Center/immunology , Germinal Center/metabolism , Haptens/immunology , Haptens/metabolism , Kinetics , Male , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Nippostrongylus/immunology , Nippostrongylus/metabolism , Plasma Cells/cytology , Plasma Cells/immunology , Plasma Cells/metabolism , Positive Regulatory Domain I-Binding Factor 1 , T-Lymphocytes/cytology , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Transcription Factors/immunology , Transcription Factors/metabolismABSTRACT
MicroRNA (miRNA)-deficient helper T cells exhibit abnormal IFN-γ production and decreased proliferation. However, the contributions of individual miRNAs to this phenotype remain poorly understood. We conducted a screen for miRNA function in primary T cells and identified individual miRNAs that rescue the defects associated with miRNA deficiency. Multiple members of the miR-17 and miR-92 families enhanced miRNA-deficient T cell proliferation whereas miR-29 largely corrected their aberrant interferon-γ (IFN-γ) expression. Repression of IFN-γ production by miR-29 involved direct targeting of both T-bet and Eomes, two transcription factors known to induce IFN-γ production. Although not usually expressed at functionally relevant amounts in helper T cells, Eomes was abundant in miRNA-deficient cells and was upregulated after miR-29 inhibition in wild-type cells. These results demonstrate that miR-29 regulates helper T cell differentiation by repressing multiple target genes, including at least two that are independently capable of inducing the T helper 1 (Th1) cell gene expression program.
Subject(s)
Interferon-gamma/metabolism , MicroRNAs/metabolism , T-Box Domain Proteins/metabolism , T-Lymphocytes, Helper-Inducer/metabolism , Animals , Cell Differentiation , Cell Proliferation , Cells, Cultured , Cytokines/genetics , Cytokines/immunology , Cytokines/metabolism , Gene Expression Regulation/immunology , Interferon-gamma/genetics , Interferon-gamma/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , MicroRNAs/genetics , MicroRNAs/immunology , Proteins/genetics , RNA-Binding Proteins , T-Box Domain Proteins/immunology , T-Lymphocytes, Helper-Inducer/cytology , T-Lymphocytes, Helper-Inducer/immunologyABSTRACT
To move directionally, cells can bias the generation of protrusions or select among randomly generated protrusions. Here we use 3D two-photon imaging of chick branchial arch 2 directed neural crest cells to probe how these mechanisms contribute to directed movement, whether a subset or the majority of cells polarize during movement, and how the different classes of protrusions relate to one another. We find that, in contrast to Xenopus, cells throughout the stream are morphologically polarized along the direction of overall stream movement and do not exhibit contact inhibition of locomotion. Instead chick neural crest cells display a progressive sharpening of the morphological polarity program. Neural crest cells have weak spatial biases in filopodia generation and lifetime. Local bursts of filopodial generation precede the generation of larger protrusions. These larger protrusions are more spatially biased than the filopodia, and the subset of protrusions that are productive for motility are the most polarized of all. Orientation rather than position is the best correlate of the protrusions that are selected for cell guidance. This progressive polarity refinement strategy may enable neural crest cells to efficiently explore their environment and migrate accurately in the face of noisy guidance cues.
Subject(s)
Cell Movement/physiology , Neural Crest/embryology , Neural Crest/physiology , Animals , Branchial Region/embryology , Cell Polarity/physiology , Chick Embryo , Chickens , Contact Inhibition , Neural Crest/metabolism , Pseudopodia/physiology , Skull/embryologyABSTRACT
Polymorphisms in genes involved in IL-4 responses segregate with allergic disease risk and correlate with IgE levels in humans, and IL-4 promotes IgE and IgG1 Ab production against allergens in mice. We report that mice with only one intact Il4 gene copy are significantly impaired in their ability to make specific IgE responses against allergens, whereas IgG1 responses to allergens remain unaffected. Il4-hemizygosity also resulted in a modest but detectable drop in IL-4 production by CD4+ T cells isolated from lymph nodes and prevented IgE-dependent oral allergen-induced diarrhea. We conclude that a state of haploinsufficiency for the Il4 gene locus is specifically relevant for IL-4-dependent IgE responses to allergens with the amount of IL-4 produced in the hemizygous condition falling close to the threshold required for switching to IgE production. These results may be relevant for how polymorphisms in genes affecting IL-4 responses influence the risk of IgE-mediated allergic disease in humans.
Subject(s)
Allergens/immunology , Haploinsufficiency , Immunoglobulin E/immunology , Interleukin-4/genetics , Animals , CD4-Positive T-Lymphocytes/immunology , Hypersensitivity/immunology , Immunoglobulin E/biosynthesis , Immunoglobulin G/immunology , Interleukin-4/immunology , Mice , Pollen/immunology , Polymorphism, GeneticABSTRACT
One of the main questions in the field of imaging immune cell migration in living tissues is whether cells fulfill their functionality via random or nonrandom migration processes. For some applications, this issue has remained controversial even after publication of various imaging studies. A prime example is B-cell migration in germinal centers (GCs) where somatic hypermutation and clonal selection of B cells are thought to occur within morphologically distinct regions termed dark zone (DZ) and light zone (LZ). Here, we reanalyze a previously published dataset on GC B-cell migration, applying a sensitive analysis technique to detect directed migration and using a procedure to correct for a number of artifacts that frequently occur in time-lapse imaging experiments. Although B cells roughly perform a persistent random walk, we present evidence that they have a small preference (of on average about 0.2-0.3 µm min(-1)) to migrate from DZ to LZ, which is consistent with classical views of the GC reaction. This preference is most pronounced among a large subset of almost half of the B-cell population migrating along relatively straight tracks. Using a computational model to generate long-lasting B-cell tracks based on the experimental motility data (including the small directional preference), we predict a time course to travel from DZ to LZ of a few hours. This is consistent with experimental observations, and we show that at the observed cellular motility such a time course cannot be explained without the small preferential migration from DZ to LZ.
Subject(s)
B-Lymphocytes/cytology , Cell Movement , Germinal Center/cytology , Computer Simulation , Humans , Kinetics , Models, Biological , Time-Lapse ImagingABSTRACT
Mechanisms that restrict class switch recombination (CSR) to IgE limit the subsequent production of IgE antibodies and therefore the development of allergic disease. Mice with impaired B cell receptor (BCR) signaling have significantly increased IgE responses, consistent with a role for BCR signaling in IgE regulation. While prior work focused on BCR signaling in IgE-expressing cells to explain these findings, it has been reported that BCR signaling can reduce CSR. Therefore, we investigated the possibility that IgE CSR might be particularly sensitive to inhibition by BCR signaling in unswitched B cells. We found that immunization of mice with high-affinity antigen resulted in reduced representation of IgE-expressing cells among germinal center B cells and plasma cells relative to a low-affinity antigen. Mechanistic experiments with cultured mouse B cells demonstrated that BCR ligands selectively inhibited IgE CSR in a dose-, affinity-, and avidity-dependent manner. Signaling via Syk was required for the inhibition of IgE CSR following BCR stimulation, whereas inhibition of the PI3K subunit p110δ increased IgE CSR independently of BCR ligation. The inhibition of IgE CSR by BCR ligands synergized with IL-21 or TGFß1. BCR ligation also inhibited CSR to IgE in human tonsillar B cells, and this inhibition was also synergistic with IL-21. These findings establish that IgE CSR is uniquely susceptible to inhibition by BCR signaling in mouse and human B cells, with important implications for the regulation and pathogenesis of allergic disease.
ABSTRACT
The proper regulation of IgE production safeguards against allergic disease, highlighting the importance of mechanisms that restrict IgE plasma cell (PC) survival. IgE PCs have unusually high surface B cell receptor (BCR) expression, yet the functional consequences of ligating this receptor are unknown. Here, we found that BCR ligation induced BCR signaling in IgE PCs followed by their elimination. In cell culture, exposure of IgE PCs to cognate antigen or anti-BCR antibodies induced apoptosis. IgE PC depletion correlated with the affinity, avidity, amount, and duration of antigen exposure and required the BCR signalosome components Syk, BLNK, and PLCγ2. In mice with a PC-specific impairment of BCR signaling, the abundance of IgE PCs was selectively increased. Conversely, BCR ligation by injection of cognate antigen or anti-IgE depleted IgE PCs. These findings establish a mechanism for the elimination of IgE PCs through BCR ligation. This has important implications for allergen tolerance and immunotherapy as well as anti-IgE monoclonal antibody treatments.
Subject(s)
Hypersensitivity , Plasma Cells , Animals , Mice , Apoptosis , Cell Nucleus , Cell Survival , Immunosuppressive Agents , Receptors, Antigen, B-Cell/immunologyABSTRACT
In allergic asthma, allergen inhalation leads to local Th2 cell activation and peribronchial inflammation. However, the mechanisms for local antigen capture and presentation remain unclear. By two-photon microscopy of the mouse lung, we established that soluble antigens in the bronchial airway lumen were efficiently captured and presented by a population of CD11c+ interstitial macrophages with high CX3CR1-GFP and MHC class II expression. We refer to these cells as Bronchus-Associated Macrophages (BAMs) based on their localization underneath the bronchial epithelium. BAMs were enriched in collagen-rich regions near some airway branchpoints, where inhaled antigens are likely to deposit. BAMs engaged in extended interactions with effector Th2 cells and promoted Th2 cytokine production. BAMs were also often in contact with dendritic cells (DCs). After exposure to inflammatory stimuli, DCs migrated to draining lymph nodes, whereas BAMs remained lung resident. We propose that BAMs act as local antigen presenting cells in the lung and also transfer antigen to DCs.
Subject(s)
Dendritic Cells , Th2 Cells , Allergens , Animals , Bronchi , Cytokines , Lung/pathology , Macrophages , MiceABSTRACT
Macrophages are paracrine signalers that regulate tissular responses to injury through interactions with parenchymal cells. Connexin hemichannels have recently been shown to mediate efflux of ATP by macrophages, with resulting cytosolic calcium responses in adjacent cells. Here we report that lung macrophages with deletion of connexin 43 (MacΔCx43) had decreased ATP efflux into the extracellular space and induced a decreased cytosolic calcium response in co-cultured fibroblasts compared to WT macrophages. Furthermore, MacΔCx43 mice had decreased lung fibrosis after bleomycin-induced injury. Interrogating single cell data for human and mouse, we found that P2rx4 was the most highly expressed ATP receptor and calcium channel in lung fibroblasts and that its expression was increased in the setting of fibrosis. Fibroblast-specific deletion of P2rx4 in mice decreased lung fibrosis and collagen expression in lung fibroblasts in the bleomycin model. Taken together, these studies reveal a Cx43-dependent profibrotic effect of lung macrophages and support development of fibroblast P2rx4 as a therapeutic target for lung fibrosis.
Subject(s)
Connexin 43 , Idiopathic Pulmonary Fibrosis , Adenosine Triphosphate/metabolism , Animals , Bleomycin/pharmacology , Calcium/metabolism , Connexin 43/genetics , Connexin 43/metabolism , Fibroblasts/metabolism , Idiopathic Pulmonary Fibrosis/metabolism , Macrophages/metabolism , Mice , Mice, KnockoutABSTRACT
Dendritic cells (DCs) are crucial for initiating adaptive immune responses. However, the factors that control DC positioning and homeostasis are incompletely understood. We found that type-2 conventional DCs (cDC2s) in the spleen depend on Gα13 and adhesion G protein-coupled receptor family member-E5 (Adgre5, or CD97) for positioning in blood-exposed locations. CD97 function required its autoproteolytic cleavage. CD55 is a CD97 ligand, and cDC2 interaction with CD55-expressing red blood cells (RBCs) under shear stress conditions caused extraction of the regulatory CD97 N-terminal fragment. Deficiency in CD55-CD97 signaling led to loss of splenic cDC2s into the circulation and defective lymphocyte responses to blood-borne antigens. Thus, CD97 mechanosensing of RBCs establishes a migration and gene expression program that optimizes the antigen capture and presentation functions of splenic cDC2s.
Subject(s)
Dendritic Cells/physiology , Erythrocytes/physiology , Receptors, G-Protein-Coupled/metabolism , Spleen/cytology , Spleen/immunology , Actins/metabolism , Animals , Antigen Presentation , Antigens/immunology , Blood Circulation , CD55 Antigens/blood , CD55 Antigens/metabolism , Cell Movement , Dendritic Cells/immunology , Erythrocytes/metabolism , GTP-Binding Protein alpha Subunits, G12-G13/metabolism , Homeostasis , Interferon Regulatory Factors/metabolism , Ligands , Mice , Receptors, G-Protein-Coupled/genetics , Signal Transduction , Spleen/blood supply , Spleen/metabolism , Transcription, Genetic , TranscriptomeABSTRACT
T cell immunoglobulin-domain and mucin-domain (TIM) proteins constitute a receptor family that was identified first on kidney and liver cells; recently it was also shown to be expressed on T cells. TIM-1 and -3 receptors denote different subsets of T cells and have distinct regulatory effects on T cell function. Ferritin is a spherical protein complex that is formed by 24 subunits of H- and L-ferritin. Ferritin stores iron atoms intracellularly, but it also circulates. H-ferritin, but not L-ferritin, shows saturable binding to subsets of human T and B cells, and its expression is increased in response to inflammation. We demonstrate that mouse TIM-2 is expressed on all splenic B cells, with increased levels on germinal center B cells. TIM-2 also is expressed in the liver, especially in bile duct epithelial cells, and in renal tubule cells. We further demonstrate that TIM-2 is a receptor for H-ferritin, but not for L-ferritin, and expression of TIM-2 permits the cellular uptake of H-ferritin into endosomes. This is the first identification of a receptor for ferritin and reveals a new role for TIM-2.
Subject(s)
B-Lymphocytes/metabolism , Endocytosis/immunology , Ferritins/metabolism , Kidney/metabolism , Liver/metabolism , Membrane Proteins/metabolism , Animals , Antibodies, Monoclonal , Cell Line, Tumor , Cloning, Molecular , DNA Primers , Flow Cytometry , Fluorescent Antibody Technique , Green Fluorescent Proteins , Immunohistochemistry , Mice , Mice, Inbred C57BL , Polymerase Chain ReactionABSTRACT
Stringent regulation of IgE antibody production is critical for constraining allergic responses. This review discusses recent advances in understanding cell-intrinsic and extrinsic mechanisms that regulate the genesis and fate of IgE B cells. B cell-intrinsic regulation of IgE is orchestrated by the IgE B Cell Receptor (BCR). Through its antigen-independent signaling and low surface expression, the IgE BCR drives IgE B cells to differentiate into short-lived plasma cells and/or undergo apoptosis, restricting IgE-expressing cells from entering long-lived compartments. The pivotal extrinsic regulators of IgE responses are T follicular helper cells (TFH). TFH produce IL-4 and IL-21, which, respectively, are the major activating and inhibitory cytokines for IgE class-switching. Other newly identified T follicular subsets also contribute to IgE regulation. Although IgE responses are normally constrained, recent studies suggest that specific conditions can induce the formation of IgE responses with enhanced affinity or longevity, effectively 'breaking the rules' of IgE regulation.
Subject(s)
Antibody Formation/immunology , B-Lymphocytes/immunology , Immunoglobulin E/immunology , Immunomodulation , Animals , Antibody Formation/genetics , Apoptosis/genetics , Apoptosis/immunology , B-Lymphocytes/metabolism , Cytokines/metabolism , Disease Susceptibility , Gene Expression Regulation , Germinal Center/immunology , Germinal Center/metabolism , Humans , Hypersensitivity/immunology , Hypersensitivity/metabolism , Immunologic Memory , Plasma Cells/immunology , Plasma Cells/metabolism , Receptors, Antigen, B-Cell/genetics , Receptors, Antigen, B-Cell/metabolismABSTRACT
MicroRNAs (miRNAs, miRs) regulate cell fate decisions by post-transcriptionally tuning networks of mRNA targets. We used miRNA-directed pathway discovery to reveal a regulatory circuit that influences Ig class switch recombination (CSR). We developed a system to deplete mature, activated B cells of miRNAs, and performed a rescue screen that identified the miR-221/222 family as a positive regulator of CSR. Endogenous miR-221/222 regulated B cell CSR to IgE and IgG1 in vitro, and miR-221/222-deficient mice exhibited defective IgE production in allergic airway challenge and polyclonal B cell activation models in vivo. We combined comparative Ago2-HITS-CLIP and gene expression analyses to identify mRNAs bound and regulated by miR-221/222 in primary B cells. Interrogation of these putative direct targets uncovered functionally relevant downstream genes. Genetic depletion or pharmacological inhibition of Foxp1 and Arid1a confirmed their roles as key modulators of CSR to IgE and IgG1.