ABSTRACT
As a traditional herbal medicine and food in China and many other Asian countries, the areca nut (Areca catechu L.) is not only widely used for the treatment of various diseases, but also popular as a chewing hobby. However, as a first-class carcinogen designated by IARC, clinical studies have shown that long-term chewing of areca nut is associated with oral mucosal diseases and even oral cancer. Moreover, the incidence of these diseases varies regionally, suggesting that it may be related to edible methods in different regions. In this study, UPLC-Q-TOF-MSE was combined with feature-based molecular networking to systematically characterise the chemical ingredients of areca nut. Based on these results, the ingredients of different edible parts and edible methods was rapidly compared. The compositional changes during the production process were also analysed. The obtained results provide a foundation for the scientific utilisation of areca nut.
Subject(s)
Areca , Plants, Medicinal , Mastication , Nuts , AsiaABSTRACT
In this study, CH4 production from anaerobic digestion (AD) of refractory cellulose was investigated at a high loading of 3.5 (VScellulose/VSinoculum) under nanobubble water (NBW) addition. A longer proton spin-spin relaxation time (2611-2906 ms) of NBW during 35 days' storage reflected its high mobility and diffusion of water molecules. Higher volatile fatty acids were yielded at the hydrolysis-acidification stage under NBW addition. Methanogenesis tests showed that Air-NBW and CO2-NBW supplementation accelerated the utilization of crystalline cellulose, achieving methane yields of 264 and 246 mL CH4/g-VSreduced, increasing by 18% and 10% compared to deionized water addition (the control), respectively. In addition, under NBW addition the cellulose crystallinity reduction was enhanced by 14-20% with microbial community being enriched with hydrolytic and methanogenic bacteria. Results from this work suggest that NBW environment with no chemical addition and relatively low energy consumption is advantageous for enhanced AD process of cellulosic biomass.
Subject(s)
Bioreactors , Cellulose , Anaerobiosis , Dietary Supplements , Hydrogen-Ion Concentration , Hydrolysis , Methane , WaterABSTRACT
3-n-Butylphthalide (NBP) [(±)-3-butyl-1(3H)-isobenzofuranone] is an anti-cerebral-ischemia drug. Moderate hepatotoxicity has been observed in clinical applications. One of the major metabolites, 3-N-acetylcysteine-NBP, has been detected in human urine, indicating the formation of a reactive metabolite. We elucidated the formation mechanism of the reactive metabolite and its association with the hepatotoxicity of NBP. The in vitro incubations revealed that 3-glutathione-NBP (3-GSH-NBP) was observed only in fresh rat liver homogenate rather than in liver microsomes, liver cytosol, or liver 9,000g supernatant supplemented with NADPH and GSH. We also detected 3-GSH-NBP when 3'-phosphoadenosine-5'-phosphosulfate was added in GSH-fortified human liver cytosol (HLC). The formation of 3-GSH-NBP was 39.3-fold higher using 3-hydroxy-NBP (3-OH-NBP) as the substrate than NBP. The sulfotransferase (SULT) inhibitors DCNP (2,6-dichloro-4-nitrophenol) and quercetin suppressed 3-GSH-NBP formation in HLC by 75 and 82%, respectively, suggesting that 3-OH-NBP sulfation was involved in 3-GSH-NBP formation. Further SULT phenotyping revealed that SULT1A1 is the major isoform responsible for the sulfation. Dose-dependent toxicity was observed in primary rat hepatocytes exposed to 3-OH-NBP, with an IC50 of approximately 168 µM. Addition of DCNP and quercetin significantly increased cell viability, whereas l-buthionine-sulfoximine (a GSH depleter) decreased cell viability. Overall, our study revealed the underlying mechanism for the bioactivation of NBP is as follows. NBP is first oxidized to 3-OH-NBP and further undergoes sulfation to form 3-OH-NBP sulfate. The sulfate spontaneously cleaves off, generating highly reactive electrophilic cations, which can bind either to GSH to detoxify or to hepatocellular proteins to cause undesirable side effects.
Subject(s)
Arylsulfotransferase/metabolism , Benzofurans/metabolism , Neuroprotective Agents/metabolism , Sulfur Compounds/metabolism , Acetylcysteine/metabolism , Animals , Benzofurans/pharmacokinetics , Benzofurans/toxicity , Biotransformation , Cell Survival/drug effects , Cells, Cultured , Cytosol/enzymology , Cytosol/metabolism , Dose-Response Relationship, Drug , Glutathione/metabolism , Hepatocytes/drug effects , Humans , Hydroxylation , Kidney/enzymology , Kidney/metabolism , Liver/enzymology , Liver/metabolism , Male , Microsomes, Liver/enzymology , Microsomes, Liver/metabolism , Neuroprotective Agents/pharmacokinetics , Neuroprotective Agents/toxicity , Rats , Rats, Sprague-DawleyABSTRACT
Scutellarin [scutellarein-7-O-glucuronide (S-7-G)] displayed a unique pharmacokinetic profile in humans after oral administration: the original compound was hardly detected, whereas its isomeric metabolite isoscutellarin [scutellarein-6-O-glucuronide (S-6-G)] had a markedly high exposure. Previous rat study revealed that S-7-G and S-6-G in the blood mainly originated from their aglycone in enterocytes, and that the S-7-G/S-6-G ratio declined dramatically because of a higher hepatic elimination of S-7-G. In the present study, metabolite profiling in human excreta demonstrated that the major metabolic pathway for S-6-G and S-7-G was through further glucuronidation. To further understand the cause for the exposure difference between S-7-G and S-6-G in humans, studies were conducted to uncover mechanisms underlying their formation and elimination. In vitro metabolism study suggested that S-7-G was formed more easily but metabolized more slowly in human intestinal and hepatic microsomes. Efflux transporter study showed that S-6-G and S-7-G were good substrates of breast cancer resistance protein and multidrug resistance-associated protein (MRP) 2 and possible substrates of MRP3; however, there was no preference great enough to alter the S-7-G/S-6-G ratio in the blood. Among the major hepatic anion uptake transporters, organic anion-transporting polypeptide (OATP) 2B1 played a predominant role in the hepatic uptake of S-6-G and S-7-G and showed greater preference for S-7-G with higher affinity than S-6-G (K(m) values were 1.77 and 43.9 µM, respectively). Considering the low intrinsic permeability of S-6-G and S-7-G and the role of OATP2B1 in the hepatic clearance of such compounds, the selective hepatic uptake of S-7-G mediated by OATP2B1 is likely a key determinant for the much lower systemic exposure of S-7-G than S-6-G in humans.
Subject(s)
Apigenin/pharmacokinetics , Drugs, Chinese Herbal/pharmacokinetics , Glucuronates/pharmacokinetics , Intestinal Absorption , Intestinal Mucosa/metabolism , Liver/metabolism , Organic Anion Transporters/metabolism , Administration, Oral , Adult , Apigenin/administration & dosage , Apigenin/blood , Apigenin/urine , Bile/metabolism , Biotransformation , Drugs, Chinese Herbal/administration & dosage , Female , Flavones/pharmacokinetics , Glucuronates/administration & dosage , Glucuronates/blood , Glucuronates/urine , Glucuronides/metabolism , Glucuronosyltransferase/pharmacokinetics , HEK293 Cells , Humans , Male , Metabolic Clearance Rate , Metabolomics/methods , Microsomes, Liver/metabolism , Middle Aged , Multidrug Resistance-Associated Protein 2 , Organic Anion Transporters/genetics , Permeability , TransfectionABSTRACT
Thirty-six naturally occurring compounds, including four C(10)-acetylenic glycosides and a lignan, were isolated from the whole plants of Saussurea cordifolia. Their structures were elucidated by means of spectroscopic and chemical methods to be 4,6-decadiyne-1-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (1), 4,6-decadiyne-1-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (2), (8E)-decaene-4, 6-diyn-1-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (3), (8Z)-decaene-4,6-diyn-1-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (4), and (2R,3S,4S)-4-(4-hydroxy-3-methoxybenzyl)-2-(5-hydroxy-3-methoxyphenyl)-3-(hydroxymethyl)-tetrahydrofuran-3-ol (5).