ABSTRACT
PURPOSE: The hypothesis was tested that coffee types differing in content of major constituents also differ with regard to cardiometabolic effects. METHODS: Overweight persons (n = 118) were randomized to consume a dark roast [rich in N-methylpyridinium (NMP)] or medium roast (rich in caffeoylquinic acids, trigonelline) coffee blend for 3 months, after a washout period of 4 weeks. Before and after the intervention period, body weight and 15 further general and biochemical parameters were determined. RESULTS: Participants consumed an average of 4-5 cups per day. Mean body weight, body mass index and waist circumference did not change during the coffee consumption phase in either of the study groups. Systolic blood pressure decreased in the dark roast coffee group only (p < 0.05). High-density lipoprotein cholesterol levels increased in the medium roast coffee group only, and triglyceride levels increased in the dark roast coffee group only. Glucoregulation and insulin levels were not affected, although there was a small increase of hemoglobin A1c values in both groups. An increase of adiponectin levels occurred in the medium roast coffee group only and was negatively associated with NMP concentrations. Differences did not remain statistically significant after correction for multiple testing. CONCLUSIONS: Medium and dark roast coffee blends exert small but possibly relevant different cardiometabolic effects. Further studies of health outcomes in relation to coffee constituents seem warranted.
Subject(s)
Cardiovascular System/drug effects , Coffee/chemistry , Overweight/metabolism , Adiponectin/blood , Adolescent , Adult , Aged , Alkaloids/administration & dosage , Blood Glucose/metabolism , Blood Pressure/drug effects , Body Mass Index , Body Weight , C-Reactive Protein , Cardiovascular System/metabolism , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Fasting , Female , Glycated Hemoglobin/metabolism , Humans , Insulin/blood , Linear Models , Male , Middle Aged , Osteopontin/blood , Prospective Studies , Pyridinium Compounds/administration & dosage , Pyridinium Compounds/blood , Quinic Acid/administration & dosage , Quinic Acid/analogs & derivatives , Waist Circumference , Weight Loss/drug effects , Young AdultABSTRACT
Coffee consumption sometimes is associated with symptoms of stomach discomfort. This work aimed to elucidate whether two coffee beverages, containing similar amounts of caffeine, but differing in their concentrations of (ß) N-alkanoyl-5-hydroxytryptamides (C5HTs), chlorogenic acids (CGAs), trigonelline, and N-methylpyridinium (N-MP) have different effects on gastric acid secretion in healthy volunteers. The intragastric pH after administration of bicarbonate with/without 200 mL of a coffee beverage prepared from a market blend or dark roast blend was analyzed in nine healthy volunteers. Coffee beverages were analyzed for their contents of C5HT, N-MP, trigonelline, CGAs, and caffeine using HPLC-DAD and HPLC-MS/MS. Chemical analysis revealed higher concentrations of N-MP for the dark brown blend (87 mg/L) compared to the market blend coffee (29 mg/L), whereas concentrations of C5HT (0.012 versus 0.343 mg/L), CGAs (323 versus 1126 mg/L), and trigonelline (119 versus 343 mg/L) were lower, and caffeine concentrations were similar (607 versus 674 mg/mL). Gastric acid secretion was less effectively stimulated after administration of the dark roast blend coffee compared to the market blend. Future studies are warranted to verify whether a high ratio of N-MP to C5HT and CGAs is beneficial for reducing coffee-associated gastric acid secretion.
Subject(s)
Coffee/chemistry , Gastric Acid/metabolism , Adult , Alkaloids/administration & dosage , Alkaloids/analysis , Caffeine/administration & dosage , Caffeine/analysis , Chlorogenic Acid/administration & dosage , Chlorogenic Acid/analysis , Chromatography, High Pressure Liquid , Double-Blind Method , Female , Healthy Volunteers , Humans , Hydrogen-Ion Concentration , Male , Niacinamide/administration & dosage , Niacinamide/analogs & derivatives , Niacinamide/analysis , Pyridinium Compounds/administration & dosage , Pyridinium Compounds/analysis , Tandem Mass Spectrometry , Tryptamines/administration & dosage , Tryptamines/analysis , Young AdultABSTRACT
This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.
Subject(s)
Chlorogenic Acid/analogs & derivatives , Coffee/chemistry , NF-E2-Related Factor 2/genetics , Pyridinium Compounds/pharmacology , Quinic Acid/analogs & derivatives , Chlorogenic Acid/pharmacology , Gene Expression Regulation/drug effects , Glutathione Transferase/genetics , HT29 Cells/drug effects , Humans , Lymphocytes/drug effects , NAD(P)H Dehydrogenase (Quinone)/genetics , NF-E2-Related Factor 2/metabolism , Polymorphism, Single Nucleotide , Protein Transport/drug effects , Quinic Acid/pharmacology , Response ElementsABSTRACT
Recent results from prospective cohort studies have shown that moderate coffee consumption is associated with a reduced risk for diabetes mellitus type II or Alzheimer's disease. Since reactive oxygen species (ROS) are believed to be involved in the pathogenesis of these diseases, antioxidants in coffee might contribute to this risk reduction. We aimed at elucidating whether a dark roast coffee beverage (CB) rich in N-methylpyridinium ions (NMP: 785 µmol/L) and low in chlorogenic acids (CGA: 523 µmol/L) has stronger antioxidant effects on human erythrocytes than a CB prepared from a light roast with opposite proportions (CGA: 4538 µmol/L; NMP: 56 µmol/L). Following a 2-wk wash out period, 500 mL of the respective CB was administered to 30 subjects daily for 4-wk. Blood and spot urine samples were collected at the beginning and at the end of each intervention. Intake of the dark roast CB most effectively improved the antioxidant status of erythrocytes: superoxide dismutase and glutathione peroxidase activity decreased by 5.8 and 15%, respectively, whereas tocopherol and total glutathione concentrations increased by 41 and 14%, respectively. Furthermore, administration of the NMP-rich CB led to a significant body weight reduction in pre-obese subjects, whereas the CGA-rich CB did not.
Subject(s)
Body Weight/drug effects , Coffee , Glutathione/blood , Pyridinium Compounds/pharmacology , Vitamin E/blood , Antioxidants/metabolism , Chlorogenic Acid/chemistry , Coffee/chemistry , Cooking , Erythrocytes/metabolism , Glutathione Peroxidase/blood , Humans , Pyridinium Compounds/chemistry , Superoxide Dismutase/bloodABSTRACT
Epidemiological and experimental evidence increasingly suggests coffee consumption to be correlated to prevention or delay of degenerative diseases connected with oxidative cellular stress. In an intervention study comprising 33 healthy volunteers, we examined DNA-protective and antioxidative effects exerted in vivo by daily ingestion of 750 mL of freshly brewed coffee rich in both green coffee bean constituents as well as roast products. The study design encompassed an initial 4 wk of wash-out, followed by 4 wk of coffee intake and 4 wk of second wash-out. At the start and after each study phase blood samples were taken to monitor biomarkers of oxidative stress response. In addition, body weight/composition and intake of energy/nutrients were recorded. In the coffee ingestion period, the primary endpoint, oxidative DNA damage as measured by the Comet assay (± FPG), was markedly reduced (p<0.001). Glutathione level (p<0.05) and GSR-activity (p<0.01) were elevated. Body weight (p<0.01)/body fat (p<0.05) and energy (p<0.001)/nutrient (p<0.001-0.05) intake were reduced. Our results allow to conclude that daily consumption of 3-4 cups of brew from a special Arabica coffee exerts health beneficial effects, as evidenced by reduced oxidative damage, body fat mass and energy/nutrient uptake.
Subject(s)
Antioxidants/pharmacology , Body Weight , Coffee , DNA Damage , Glutathione/metabolism , Adult , Body Composition , Glutathione Reductase/metabolism , Humans , MaleABSTRACT
Recently, the coffee constituents 5-O-caffeoylquinic acid (CGA) and N-methylpyridinium (NMP) were identified as inducers of the Nrf2/antioxidant-response element (ARE) detoxifying pathway under cell-culture condition. To study the impact of CGA and NMP on the Nrf2-activating properties of a complex coffee beverage, two different model coffees were generated by variation of the roasting conditions: a low-roast coffee rich in CGA and a heavy-roast low in CGA but containing high levels of NMP. Activation of the Nrf2/antioxidant-response element pathway was monitored in vitro and in vivo.
Subject(s)
Antioxidants/pharmacology , Chemoprevention , Chlorogenic Acid/pharmacology , Coffee/chemistry , NF-E2-Related Factor 2/physiology , Pyridinium Compounds/pharmacology , Response Elements/physiology , Enzyme Induction/drug effects , Glutamate-Cysteine Ligase/biosynthesis , HT29 Cells , Heme Oxygenase-1/biosynthesis , Humans , Reactive Oxygen Species/metabolismABSTRACT
SCOPE: Epidemiological studies suggest that coffee can reduce the risk of degenerative diseases such as diabetes type 2, cardiovascular disease and cancer. These beneficial effects have partly been attributed to the antioxidant activity of coffee. We determined composition and antioxidant potential of differentially roasted coffee extracts and investigated the impact of selected original constituents and roast products. METHODS AND RESULTS: Parameters studied were direct antioxidant activity (trolox equivalent antioxidant capacity/oxygen radical absorbing capacity), cellular reactive oxygen species (ROS) level, DNA damage and protein expression of NAD(P)H: quinone oxidoreductase, γ-glutamylcysteine ligase and glutathione reductase in HT-29/Caco-2 cells at 24-h incubation. All extracts showed distinct direct antioxidant activity: medium roasts>light roast AB1 (caffeoylquinic acid (CQA)-rich Arabica Brazil extract); dark roast AB2 (N-methylpyridinium (NMP)-rich Arabica Brazil extract), and diminished t-butylhydroperoxide-induced ROS level in HT-29 cells (AB2>medium roasts>AB1). NAD(P)H:quinone oxidoreductase 1 expression and γ-glutamylcysteine ligase expression were distinctly induced by AB1 and 5-CQA, but not by AB2 and NMP. 5-CQA and caffeic acid exhibited highest trolox equivalent antioxidant capacity/oxygen radical absorbing capacity values (5-CQA: 1.3/3.5 mM and caffeic acid: 1.3/3.9 mM trolox); ROS level was distinctly diminished by 5-CQA (≥3 µM), catechol (30 µM) and trigonelline (≥30 µM), whereas menadione-induced DNA damage in Caco-2 cells was reduced by NMP compounds (1-30 µM). CONCLUSION: The results emphasize that both original constituents and roast products contribute to the cellular antioxidant effectiveness of coffee.