ABSTRACT
ObjectiveTo investigate the mechanism of Jiedu Huoxue prescription in promoting the reendothelialization of injured vessels by regulating the nuclear factor （NF）-κB/NOD-like receptor protein 3 （NLRP3）/cysteine-aspartic acid protease （Caspase）-1-mediated pyroptosis. MethodA rat model of injured thoracic aorta was established by balloon injury， and 36 rats were assigned into shame surgery， model， low-， medium-， and high-dose Jiedu Huoxue prescription， and atorvastatin calcium tablet groups. The injured aortic segment was collected 28 days after surgery. Hematoxylin-eosin （HE） staining and Evans blue staining were conducted to reveal the changes of vascular structural morphology and the reendothelialization of blood vessels， respectively. The enzyme-linked immunosorbent assay （ELISA） was employed to determine the levels of tumor necrosis factor-α （TNF-α）， intercellular adhesion molecule-1 （ICAM-1）， interleukin （IL）-1β， and nitric oxide （NO） in the serum. Western blotting was employed to determine the expression of endothelial nitric oxide synthase （eNOS）， NF-κB p65， phospho-NF-κB p65 （p-NF-κB p65）， NLRP3， and Caspase-1 in the vascular tissue. ResultThe model group showed thickened endovascular membrane， proliferation and disarrangement of smooth muscle cells of the artery wall， obvious inflammatory cell infiltration， and narrowed luminal area. Jiedu Huoxue prescription and atorvastatin calcium tablets mitigated the pathological changes of the thoracic aorta in different degrees. After balloon injury， the endothelial coverage rate of the model group decreased significantly， while Jiedu Huoxue prescription and atorvastatin calcium tablets increased the reendothelialization rate （P<0.05）. Compared with the shame surgery group， the model group showed elevated levels of TNF-α， ICAM-1， and IL-1β （P<0.01） and lowered NO level （P<0.01） in the serum. In addition， the model group presented down-regulated protein level of eNOS （P<0.01） and up-regulated phosphorylation of pyroptosis-associated proteins NLPR3， Caspase-1， and NF-κB p65 in the vascular tissue （P<0.05， P<0.01）. Compared with the model group， Jiedu Huoxue prescription and atorvastatin calcium tablets lowered TNF-α， ICAM-1， and IL-1β levels （P<0.05， P<0.01） and elevated the NO level in the serum （P<0.05， P<0.01）. Moreover， the drugs up-regulated the expression of eNOS （P<0.01） and down-regulated the expression of NLRP3， Caspase-1， and NF-κB p65 （P<0.05， P<0.01） in the vascular tissue. ConclusionJiedu Huoxue prescription can promote the reendothelialization and inhibit the intimal hyperplasia of vessels after balloon injury by regulating the NF-κB/NLRP3/Caspase-1 pathway to inhibit pyroptosis and reduce endothelial inflammatory injury.
ABSTRACT
This study was aimed to make clear the origin and clinical applications of the Tibetan medicine "Bangga".Based on the systematical consultation of the Tibetan medical literature documents and the Chinese version,such as The King's Medicine,The Four Medical Tantras,Jing Zhu Materia Medica,the herbal textual research was made on the name,based source,origin and harvesting season,function,indication and etc.of Tibetan medicine "Bangga".The results showed that Tibetan medicine "Bangga" comes from the whole dried plant of Aconitum naviculare Stapf or A.tanguticum (Maxim) Stapf of Ranunculaceae plants.It was concluded that the research on the origin of Tibetan medicine "Bangga" can provide a basis for the application and promotion of quality standards of "Bangga".
ABSTRACT
Objective To observe the clinical therapeutic efficacy of prescription of supplementing qi and activating yang on sick sinus syndrome. Methods According to the random number table, 231 patients with sick sinus syndrome were randomly divided into treatment group and control group. One hundred and fifty patients in the treatment group were given the Chinese herbal medicine while the other 81 patients in the control group were given atropine. The therapeutic course was four weeks in each group. The changes of clinical manifestations and electrocardiogram (24 h Holter monitoring ECG) of two groups were compared. Results The total effectiveness of treatment group was 98.67% (148/150), the control group was 41.98% (34/81), there was a significant difference in the clinical curative efficacy (P<0.01). The total effective rate of the treatment group was 87.3%(131/150) and that of the control group was 35.9%(29/81), and the average increases of heart rates in 24 hours were 9/min and 4/min, respectively. There was significant difference between the two groups (P <0.05). Conclusion The prescription of supplementing qi and activating yang was effective in improving the clinical symptoms and heart rates of patients with sick sinus syndrome.
ABSTRACT
Objective To observe the difference clinical therapeutic efficacy of the Chinese herbal medicine and western medicine on sick sinus syndrome patients.Method 171 patients with sick sinus syndrome were randomly divided into treatment group and control group.90 patients in the treatment group were given Qiangxin Fumai Mixture while the other 81 patients in the control group were given atropine.The therapeutic course was four weeks in each group.The changes of clinical manifestations,electrocardiogram(24 h Holter monitoring ECG) and left ventricular ejection fraction(LVEF) were compared between two groups.Result Clinical therapeutic efficacy:There was significant difference between two groups(P